PMID: 7543933Sep 1, 1995Paper

A charybdotoxin-sensitive, Ca(2+)-activated K+ channel with inward rectifying properties in brain microvascular endothelial cells: properties and activation by endothelins

Journal of Neurochemistry
C Van RenterghemC Frelin

Abstract

A charybdotoxin-sensitive, Ca(2+)-activated K+ channel was identified in cultured rat brain capillary endothelial cells by using conventional single-channel recording techniques and 86(Rb+)-influx and efflux experiments. Channel activity was dependent on the presence of Ca2+ on the cytosolic face of the membrane with a threshold concentration of 100 nM. It was inhibited by charybdotoxin (IC50 30 nM) and quinine (IC50 0.1 mM) but not by apamin. K(Ca) channels showed unusual inward rectifying properties under asymmetrical ionic conditions. They were activated by endothelin-1 (EC50 0.7 nM) and endothelin-3 (EC50 7-10 nM). The actions of endothelins were prevented by BQ-123 (Ki = 8 nM) in a competitive fashion, hence suggesting the involvement of an ETA-receptor subtype. The channel activity was unaffected by cyclic AMP- or cyclic GMP-elevating agents. The possible role of the intermediate conductance, Ca(2+)-activated K+ channels for mediating K+ movements across the blood-brain barrier is discussed.

Citations

Apr 1, 1997·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·M P DehouckC Frelin
Oct 23, 1997·Proceedings of the National Academy of Sciences of the United States of America·T M IshiiJ Maylie
Jul 17, 1999·The American Journal of Physiology·M Dittrich, J Daut
Jul 25, 2000·American Journal of Physiology. Endocrinology and Metabolism·L HertzM Spatz
May 18, 2001·American Journal of Physiology. Heart and Circulatory Physiology·H A ColemanH C Parkington
Nov 21, 2007·Pflügers Archiv : European journal of physiology·Ian D MillarStephen B Hladky
Jan 1, 1997·Annual Review of Physiology·B NiliusG Droogmans
Nov 11, 1999·American Journal of Respiratory and Critical Care Medicine·S FalkS Uhlig
Oct 3, 2001·Physiological Reviews·B Nilius, G Droogmans
Sep 12, 2013·Pflügers Archiv : European journal of physiology·Ruth MokgokongStephen B Hladky
Oct 14, 2004·Clinical and Experimental Pharmacology & Physiology·Harold A ColemanHelena C Parkington
Dec 6, 2014·Stroke; a Journal of Cerebral Circulation·Yi-Je ChenMartha E O'Donnell
Oct 19, 2017·Scientific Reports·Ingmar LautenschlägerStefan Uhlig
Jan 10, 2002·Biochimica Et Biophysica Acta·Barbara Del CarloMario Pellegrino

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