PMID: 6405791Jun 15, 1983Paper

A chemical and enzymological account of the multiple forms of human liver aldehyde dehydrogenase. Implications for ethnic differences in alcohol metabolism

Biochimica Et Biophysica Acta
G L Jones, Y S Teng

Abstract

Three major low-pI zones of aldehyde dehydrogenase (aldehyde:NAD+ oxidoreductase, EC 1.2.1.3) may be visualized with specific histochemical staining after starch gel electrophoresis at pH 7.4 of Caucasian human liver extracts, whereas about 50% of Chinese human liver extracts show only two such zones. The three zones of activity were purified to apparent homogeneity from Caucasian liver. The substrate specificity of each form was investigated by double reciprocal plots using 13 aldehydes of various chemistries. The acetaldehyde-preferring isozyme I lacking in 50% of Chinese livers had a slightly lower native and subunit molecular weight than the "universal' isozymes IIa and IIb. All forms were highly sensitive to disulfiram inhibition. This inhibition could be protected against, or reversed, by dithiothreitol. 2,2'-Dithiodipyridine was a slower inhibitor of isoenzyme I. All three purified forms of the enzyme, as well as crude extracts of normal and isozyme I-deficient Chinese livers, showed positive immunoreactivity to antibodies prepared in rabbits against type I enzyme. Tryptic peptide maps of forms IIa and IIb were almost identical, whereas that of form I, although showing some similarities, was clearly different. These resu...Continue Reading

References

Oct 2, 1979·Human Genetics·H W GoeddeD P Agarwal
Aug 1, 1976·Archives of Biochemistry and Biophysics·J H Eckfeldt, T Yonetani
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May 7, 1982·Science·R C Vallari, R Pietruszko

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Citations

Apr 5, 2002·American Journal of Medical Genetics·M Michael Cohen, Kohei Shiota
Jan 1, 1985·The International Journal of Biochemistry·E M Algar, R S Holmes
Jan 1, 1990·Pharmacology & Therapeutics·H W Goedde, D P Agarwal
Sep 4, 2014·Human Genomics·Andrew A MonteVasilis Vasiliou
Jan 13, 2015·Molecular and Cellular Endocrinology·Vladimir VukicevicMonika Ehrhart-Bornstein
Jan 11, 1992·Critical Reviews in Toxicology·R O BeauchampF W Kari
Dec 1, 1985·Journal of Neurochemistry·J A MaringR Little
May 1, 1985·Alcoholism, Clinical and Experimental Research·J A DuleyR S Holmes
Jan 1, 1999·The Journal of British Studies·F Mort
Jan 1, 1990·Zeitschrift für Rechtsmedizin. Journal of legal medicine·R EckeyH W Goedde

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