A chemiluminescence-based catalase assay using H2 O2 -sensitive CdTe quantum dots

Mikrochimica Acta
Fahimeh GhavamipourKhosro Khajeh

Abstract

A method is described for the chemiluminescence based determination of the activity of catalase (CAT) using H2O2-sensitive CdTe quantum dots (QDs). It is based on the finding that the chemiluminescence (CL) of the CdTe/H2O2 system is reduced due to the consumption of H2O2 by the catalytic action of CAT. The Michaelis constant is calculated to be 519 ± 27 mM, showing the potential of the method to accurately measure the Km compared to the standard method. The method does not require QDs to be conjugated to biological/organic molecules and therefore is considered to be a rapid and convenient method for determination of CAT in real samples. At an incubation time of 2 s, the LOD was calculated to be 4.5 unit/mL, with a linear range from 6 to 400 unit/mL. The assay is sensitive, simple, and suitable for practical applications. Graphical abstract Schematic representation of chemiluminescence-based catalase U(CAT) assay using the CdSe QD/H2O2 system. The reduction of H2O2 is reflected by the chemiluminescence of the QDs. A mechanism is put forward based on the changes in chemiluminescence intensity of the QDs by the consumption of H2O2 due to the catalytic action of CAT.

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Citations

Apr 27, 2019·Luminescence : the Journal of Biological and Chemical Luminescence·Hongjie SongYi Lv
May 25, 2020·European Journal of Applied Physiology·Franz-Xaver ReichlBurkhard Summer
Sep 9, 2020·Toxicology in Vitro : an International Journal Published in Association with BIBRA·Luís F GriloSusana P Pereira
Oct 12, 2020·Analytical Biochemistry·Mahya MotevalianReza H Sajedi
Jul 10, 2021·Colloids and Surfaces. B, Biointerfaces·Fahimeh GhavamipourReza H Sajedi
Aug 22, 2021·Analytical and Bioanalytical Chemistry·Donghao LiuHua He

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