A chemiluminescent immunosensor based on antibody immobilized carboxylic resin beads coupled with micro-bubble accelerated immunoreaction for fast flow-injection immunoassay

Biosensors & Bioelectronics
Zhanjun YangHuangxian Ju

Abstract

A novel immunoaffinity column used as an immunosensor for flow-injection chemiluminescent (CL) immunoassay was prepared by immobilizing antibody on carboxylic resin beads. The immunosensor could fast recognize and trap the immunocomplex of horseradish peroxidase (HRP)-labeled antibody and antigen, which was firstly formed with a micro-bubble accelerated pre-incubation process, to produce a sandwich immunocomplex. The HRP introduced in the immunoaffinity column could catalyze the CL reaction to produce enzyme-enhanced emission. With alpha-fetoprotein (AFP) as a mode, a flow-injection CL immunoassay was proposed. The whole assay for one sample, including the pre-incubation and the regeneration of immunoaffinity column, could be performed within 16 min. The linear range was 1.0-80 ng/ml with a correlation coefficient of 0.998 and a detection limit of 0.1 ng/ml at a signal/noise ratio of 3. The intra- and inter-assay coefficients of variation at 20 ng/ml AFP were 1.2% and 8.5%, respectively. The storage stability of the immunoaffinity column and the accuracy for sample detection were acceptable. This flexible, sensitive, low-cost, and rapid method is valuable for clinical immunoassay.

Citations

Jun 18, 2009·Chemical Communications : Chem Comm·Liyuan ChenSongqin Liu
Jan 13, 2010·Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry·Supaporn Kradtap HartwellKate Grudpan
Aug 18, 2010·Clinica Chimica Acta; International Journal of Clinical Chemistry·Liyuan ChenSongqin Liu
Jun 26, 2017·Auto- Immunity Highlights·Luigi CinquantaNicola Bizzaro
Jun 9, 2019·Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy·Xinchun ZhouJianguo Li
Jan 15, 2009·Analytical Chemistry·Yafeng WuSongqin Liu

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