DOI: 10.1101/463109Nov 8, 2018Paper

A chemogenetic approach for optical monitoring of voltage in neurons

BioRxiv : the Preprint Server for Biology
Mayya SundukovaPaul A Heppenstall


Optical monitoring of neuronal voltage using fluorescent indicators is a powerful approach for interrogation of the cellular and molecular logic of the nervous system. Here we describe a Semisynthetic Tethered Voltage Indicator (STeVI1) based upon Nile Red that displays voltage sensitivity when genetically targeted to neuronal membranes. This environmentally sensitive probe allows for wash-free imaging and faithfully detects supra- and subthreshold activity in neurons.

Methods Mentioned

fluorescence imaging

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