Nov 6, 2018

A chemogenetic approach for optical monitoring of voltage in neurons

BioRxiv : the Preprint Server for Biology
Mayya SundukovaPaul A Heppenstall

Abstract

Optical monitoring of neuronal voltage using fluorescent indicators is a powerful approach for interrogation of the cellular and molecular logic of the nervous system. Here we describe a Semisynthetic Tethered Voltage Indicator (STeVI1) based upon Nile Red that displays voltage sensitivity when genetically targeted to neuronal membranes. This environmentally sensitive probe allows for wash-free imaging and faithfully detects supra- and subthreshold activity in neurons.

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Mentioned in this Paper

Fluorescent Dyes
Nile red
Neuronal Cell Body Membrane
Tissue Membrane
Entire Nervous System
Neurons
HAP1
Fluorescent stain
Neuronal
Nervous System Structure

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