A chemometric study of active parameters and their interaction effects in a nebulized sheath-liquid electrospray interface for capillary electrophoresis-mass spectrometry

Electrophoresis
Sara L NilssonKarin E Markides

Abstract

A chemometrics approach has been used for evaluating the effect of four experimental parameters when coupling capillary electrophoresis (CE) to electrospray ionization-mass spectrometry (ESI-MS). Electrospray voltage, sheath-liquid flow rate, nebulizing gas flow rate, and spray needle position in respect to the MS orifice were varied according to a full factorial design. In addition to main effects, two interaction effects could be identified as significant when measuring the peak intensity of the analytes, from a sample mixture containing peptides and pharmaceuticals. The first interaction effects, between the nebulizing gas flow rate and the sheath-liquid flow rate, and the second interaction effect, between the nebulizing gas flow rate and the spray position, could further explain the impact that these variables have on the spray performance. The number of theoretical plates and the baseline noise were also measured. The sheath-liquid flow was found to significantly affect the separation efficiency, while the noise level mainly was controlled by the nebulizing gas flow. The same factorial design was also used for a CE capillary with lower internal diameter (ID) and the effects of the same variables were compared on those cap...Continue Reading

Citations

Mar 20, 2013·Chemical Reviews·Naomi L Kuehnbaum, Philip Britz-McKibbin
Apr 23, 2014·Journal of Chromatography. a·Tim J CausonChristian W Klampfl
Jan 27, 2005·Journal of Mass Spectrometry : JMS
Jan 16, 2015·Mass Spectrometry Reviews·Tiago F JorgeCarla António
Mar 19, 2005·Electrophoresis·Philippe Schmitt-Kopplin, Matthias Englmann
Jul 15, 2005·Journal of Separation Science·Javier Hernández-BorgesAlejandro Cifuentes
Oct 27, 2007·Electrophoresis·Guillaume L ErnyAlejandro Cifuentes
Apr 15, 2008·Electrophoresis·Miguel HerreroAlejandro Cifuentes
Aug 27, 2010·Rapid Communications in Mass Spectrometry : RCM·Roza WojcikNorman J Dovichi
Jun 11, 2010·Journal of Separation Science·Claudia DesiderioMassimo Castagnola
Apr 18, 2006·Journal of Chromatography. a·Fernando BenaventeVictoria Sanz-Nebot
Sep 21, 2016·Philosophical Transactions. Series A, Mathematical, Physical, and Engineering Sciences·Tiago F JorgeCarla António
Jan 30, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Samuel Bernardo-BermejoMaría Luisa Marina

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