PMID: 3753663Feb 14, 1986Paper

A chicken-yeast chimeric beta-tubulin protein is incorporated into mouse microtubules in vivo

Cell
J F BondF Solomon

Abstract

The role of divergent primary sequences in restricting tubulin function was tested in vivo by a gene transfection experiment. A chicken-yeast chimeric beta-tubulin DNA was introduced into 3T3 cells using the transfection vector pSV2. The 5' end of this gene, from chicken, is similar but not identical with that of mouse beta-tubulins; the 3' end, from yeast, contains a carboxyl terminus that is very different from other known beta-tubulin sequences. The chimeric protein is incorporated efficiently into each of the microtubule structures and each of the microtubules in the host cells. The presence of the protein has no apparent effect on either growth rate or cell morphology. The results suggest that the divergent sequences in this chimeric tubulin molecule place no restrictions on its activities in mouse cells.

Citations

Jan 1, 1996·Cell Motility and the Cytoskeleton·C Gonzalez-Agosti, F Solomon
May 1, 1999·Cell Motility and the Cytoskeleton·C WalssR F Ludueña
Jan 1, 1990·Cell Motility and the Cytoskeleton·T Stearns
Aug 1, 1988·Molecular & General Genetics : MGG·J F Harper, W Mages
Apr 1, 1990·Brain Research. Developmental Brain Research·J M Gossels, V M Ingram
Nov 1, 1989·Brain Research. Molecular Brain Research·P FernyhoughD N Ishii
Jan 1, 1988·Comparative Biochemistry and Physiology. B, Comparative Biochemistry·M Little, T Seehaus
Mar 11, 1999·Micron : the International Research and Review Journal for Microscopy·I BuñoJ C Stockert
Nov 17, 2011·Nature Reviews. Molecular Cell Biology·Carsten Janke, Jeannette Chloë Bulinski
Jun 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·K F Sullivan, D W Cleveland
Apr 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·L Pillus, F Solomon
Feb 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·A SchniekeR Jaenisch
Oct 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·T N Davis, J Thorner
Mar 15, 1994·Proceedings of the National Academy of Sciences of the United States of America·H Friedman, M Snyder
Mar 29, 1994·Proceedings of the National Academy of Sciences of the United States of America·D MiklosB Barrell
Mar 14, 2009·The Journal of Biological Chemistry·Rajat Bhattacharya, Fernando Cabral
Mar 1, 1987·The Journal of Cell Biology·D W Cleveland
Apr 1, 1988·The Journal of Cell Biology·B W Schäfer, J C Perriard
Jun 1, 1988·The Journal of Cell Biology·T C HuffakerD Botstein
Apr 1, 1989·The Journal of Cell Biology·M Snyder
Aug 1, 1989·The Journal of Cell Biology·H C Joshi, D W Cleveland
Jan 1, 1990·The Journal of Cell Biology·H N BakerD B Murphy
Sep 1, 1990·The Journal of Cell Biology·H D Hoyle, E C Raff
Aug 1, 1992·The Journal of Cell Biology·T N Davis
Jan 19, 2000·Molecular Biology of the Cell·K G KozminskiD G Drubin
Jan 1, 1992·Molecular Biology of the Cell·G BarnesD Botstein
May 1, 1993·Molecular Biology of the Cell·R F Ludueña
Jan 1, 1994·Molecular Biology of the Cell·R A ReijoT C Huffaker
Aug 1, 1995·Molecular Biology of the Cell·T Wang, A Bretscher

❮ Previous
Next ❯

Related Concepts

Related Feeds

Cell eTOC

Cell is a scientific journal publishing research across a broad range of disciplines within the life sciences field. Discover the latest research from Cell here.