A chinese herbal decoction, danggui buxue tang, stimulates proliferation, differentiation and gene expression of cultured osteosarcoma cells: genomic approach to reveal specific gene activation.

Evidence-based Complementary and Alternative Medicine : ECAM
Roy C Y ChoiKarl W K Tsim

Abstract

Danggui Buxue Tang (DBT), a Chinese herbal decoction used to treat ailments in women, contains Radix Astragali (Huangqi; RA) and Radix Angelicae Sinensis (Danggui; RAS). When DBT was applied onto cultured MG-63 cells, an increase of cell proliferation and differentiation of MG-63 cell were revealed: both of these effects were significantly higher in DBT than RA or RAS extract. To search for the biological markers that are specifically regulated by DBT, DNA microarray was used to reveal the gene expression profiling of DBT in MG-63 cells as compared to that of RA- or RAS-treated cells. Amongst 883 DBT-regulated genes, 403 of them are specifically regulated by DBT treatment, including CCL-2, CCL-7, CCL-8, and galectin-9. The signaling cascade of this DBT-regulated gene expression was also elucidated in cultured MG-63 cells. The current results reveal the potential usage of this herbal decoction in treating osteoporosis and suggest the uniqueness of Chinese herbal decoction that requires a well-defined formulation. The DBT-regulated genes in the culture could serve as biological responsive markers for quality assurance of the herbal preparation.

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Citations

Aug 5, 2010·Osteoporosis International : a Journal Established As Result of Cooperation Between the European Foundation for Osteoporosis and the National Osteoporosis Foundation of the USA·Y J WeiS C Hung
May 2, 2012·Chinese Medicine·Ava Jiangyang GuoKarl Wah-Keung Tsim
May 16, 2014·Evidence-based Complementary and Alternative Medicine : ECAM·Pui Hei ChanHenry Lam
Aug 6, 2014·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Hongwei WuBrigitte Kopp
Mar 30, 2016·Chemico-biological Interactions·Miranda L XuKarl W K Tsim
Jun 7, 2015·Reproduction : the Official Journal of the Society for the Study of Fertility·Na-Ra HanHyung-Min Kim
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Dec 9, 2016·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Shun-Ting ChenYu-Jen Chen
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Dec 19, 2019·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Xiang-Peng KongKarl W K Tsim
May 14, 2016·Journal of the Science of Food and Agriculture·Ema C Rosas-BurgosÁngel A Carbonell-Barrachina

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Methods Mentioned

BETA
enzyme-linked immunosorbent assay
chip
PCR
electrophoresis

Software Mentioned

Primer
Imagequant
Array Vision

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