A Chinese Herbal Preparation, Xiao-Er-An-Shen Decoction, Exerts Neuron Protection by Modulation of Differentiation and Antioxidant Activity in Cultured PC12 Cells

Evidence-based Complementary and Alternative Medicine : ECAM
Zhonggui LiJianping Chen

Abstract

Xiao-Er-An-Shen Decoction (XEASD), a Chinese herbal formula, has been used in clinic for treating insomnia and mental excitement in children and adolescents. However, less of scientific data supports its effectiveness in clinic. Here, we aim to study the role of XEASD in regulating neuron differentiation and antioxidant activity. An HPLC-MS was used to chemically standardize herbal extract of XEASD. The standardized herbal extracts of XEASD (0.3-3.0 mg/mL) were applied onto cultured PC12 cells for 48 hours. The treatment with XEASD extract induced neurite outgrowth of PC12 cells in a dose-dependent manner, having the highest response by ~50% of differentiated cells. Application of XEASD extract dose dependently stimulated expressions of NF68, NF160, and NF200 in cultured PC12 cells. Furthermore, XEASD activated the phosphorylation of cAMP responsive element binding protein on PC12 cells, the effect of which was blocked by H89, a protein kinase A inhibitor. Moreover, XEASD showed free radical scavenging activity and stimulated the transcriptional activity of ARE. These results supported the neurobeneficial effects of XEASD in the induction of neurite outgrowth and protection against oxidative stress and could be useful for neuro...Continue Reading

References

Mar 12, 2002·Methods in Enzymology·Tetsuro IshiiMasayuki Yamamoto
Oct 8, 2004·Journal of Biochemistry and Molecular Biology·Jong-Min Lee, Jeffrey A Johnson
Nov 2, 2005·The Journal of Clinical Psychiatry·Norifumi TsunoKaren Ritchie
Feb 22, 2008·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Anka D MuellerRalph E Mistlberger
Oct 14, 2008·Sleep Medicine Reviews·Peter MeerloDennis McGinty
Oct 1, 2009·Cytotechnology·Zheng-Ai ChenGuang-Xing Bian
May 12, 2010·Pediatrics·Lisa J MeltzerJodi A Mindell
Feb 9, 2011·Journal of Affective Disorders·Chiara BaglioniDieter Riemann
Mar 29, 2014·Neurological Sciences : Official Journal of the Italian Neurological Society and of the Italian Society of Clinical Neurophysiology·M Namik OztanirM Arif Aladag
Oct 26, 2016·CNS Neuroscience & Therapeutics·Maria-Cecilia LopesLee Fu-I
Jun 9, 2017·Korean journal of pediatrics·Dong Soon KimYoung Min Ahn

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Methods Mentioned

BETA
Electrophoresis
transfection

Software Mentioned

Shimadzu Mass Workstation
SPOT
SPSS
XEASD
Test

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