A chip-based potentiometric sensor for a Zika virus diagnostic using 3D surface molecular imprinting

The Analyst
Vincent RicottaMiriam Rafailovich

Abstract

The latest Zika virus (ZIKV) pandemic caused great international concern from explosively proliferating throughout the Americas. Currently, there is no vaccine to prevent Zika virus infection and available tests rely on antibodies or RNA. Unfortunately, antibody-based detection systems can result in false positive results and RNA-based detection systems are costly, time-consuming, and impractical for testing in remote regions. In this study, a potential point-of-care (POC) diagnostic system was developed using a chip-based potentiometric sensor to detect Zika virus using a 3D molecular imprinting technique. This chip-based potentiometric sensor system was able to detect 10-1 PFU mL-1 ZIKV in a buffered solution under 20 minutes without any sample manipulation. This sensor was tested against Dengue virus at clinical viral loads and showed no sign of cross-reactivity. When tested against human saliva samples containing clinical viral loads, this sensor was able to detect 10 PFU mL-1 ZIKV among the pool of bio-macromolecules. The high sensitivity and high selectivity demonstrated here proved that this lab-on-a-chip diagnostic has the potential to become a POC detection system for rapid and accurate screening of flaviviruses.

References

Jan 7, 1998·Journal of Virology·G KunoC B Cropp
Sep 1, 1952·Transactions of the Royal Society of Tropical Medicine and Hygiene·G W A DICKA J HADDOW
Mar 1, 1954·Transactions of the Royal Society of Tropical Medicine and Hygiene·F N MACNAMARA
Oct 7, 2003·Nature Structural Biology·Wei ZhangRichard J Kuhn
Apr 14, 2005·Chemical Reviews·J Christopher LoveGeorge M Whitesides
Jun 3, 2008·Biosensors & Bioelectronics·Yantian WangMiriam Rafailovich
Aug 5, 2008·Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology·Oumar FayeAmadou Alpha Sall
Aug 6, 2008·Emerging Infectious Diseases·Robert S LanciottiMark R Duffy
Jun 12, 2009·The New England Journal of Medicine·Mark R DuffyEdward B Hayes
May 3, 2011·Emerging Infectious Diseases·Brian D FoyRobert B Tesh
Jan 1, 2011·Journal of Natural Science, Biology, and Medicine·Manjul Tiwari
Sep 7, 2012·PLoS Neglected Tropical Diseases·MeiZhi Irene LiCheong-Huat Tan
Jan 23, 2013·The American Journal of Tropical Medicine and Hygiene·Sergio I de la Cruz-HernándezRosa M del Angel
Aug 13, 2013·PLoS Neglected Tropical Diseases·Pei-Sze Jeslyn WongCheong-Huat Tan
Apr 12, 2014·Euro Surveillance : Bulletin Européen Sur Les Maladies Transmissibles = European Communicable Disease Bulletin·M BesnardDidier Musso
Oct 24, 2014·Emerging Infectious Diseases·W Thane HancockMartin Bel
Dec 23, 2014·Emerging Infectious Diseases·Ann-Claire GourinatMyrielle Dupont-Rouzeyrol
Jan 28, 2015·Emerging Infectious Diseases·Didier MussoVan-Mai Cao-Lormeau
Jun 19, 2015·Euro Surveillance : Bulletin Européen Sur Les Maladies Transmissibles = European Communicable Disease Bulletin·L ZammarchiJonas Schmidt-Chanasit
Jun 24, 2015·The American Journal of Tropical Medicine and Hygiene·Rome BuathongTanarak Plipat
Feb 9, 2016·The Lancet Infectious Diseases·Constância Flávia Junqueira Ayres
Feb 11, 2016·The New England Journal of Medicine·Jernej MlakarTatjana Avšič Županc
Mar 11, 2016·Journal of Virology·Helen M Lazear, Michael S Diamond
Mar 31, 2016·The New England Journal of Medicine·Lyle R PetersenMargaret A Honein
Apr 2, 2016·Science·Devika SirohiRichard J Kuhn
Apr 19, 2016·Emerging Infectious Diseases·Barry AtkinsonRoger Hewson
Jun 5, 2016·Neurotoxicity Research·Ankit Shah, Anil Kumar
Jun 17, 2016·Analytical Chemistry·Jinzhao SongChangchun Liu
Jun 28, 2016·Emerging Infectious Diseases·Séverine MatheusDominique Rousset
Aug 20, 2016·Euro Surveillance : Bulletin Européen Sur Les Maladies Transmissibles = European Communicable Disease Bulletin·Emanuele NicastriGiuseppe Ippolito
Sep 30, 2016·The New England Journal of Medicine·Sankar SwaminathanMarc R Couturier
Dec 18, 2016·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Kate RussellJohn T Brooks
Mar 16, 2017·Journal of Medical Virology·Frederic BedinChristine Rozand
Jun 22, 2017·Expert Review of Vaccines·Anna Durbin, Annelies Wilder-Smith
Sep 21, 2018·Neurology India·Jayantee Kalita, Usha K Misra
Sep 1, 2017·Advanced Biosystems·Qisheng JiangJeremiah J Morrissey

Related Concepts

Bedside Testing
Zika Virus Disease (Disorder)
Zika virus (organism)
Adsorption
Gold
Statistical Sensitivity
Molecular Imprinting
Electrochemical Techniques
Nanochip Analytical Devices
Limit of Detection

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