A Chlamydial Plasmid-Dependent Secretion System for the Delivery of Virulence Factors to the Host Cytosol.

MBio
Lei LeiHarlan D Caldwell

Abstract

Chlamydia are obligate intracellular Gram-negative bacteria distinguished by a unique developmental biology confined within a parasitophorous vacuole termed an inclusion. The chlamydial plasmid is a central virulence factor in the pathogenesis of infection. Plasmid gene protein 4 (Pgp4) regulates the expression of plasmid gene protein 3 (Pgp3) and chromosomal glycogen synthase (GlgA), virulence factors secreted from the inclusion to the host cytosol by an unknown mechanism. Here, we identified a plasmid-dependent secretion system for the cytosolic delivery of Pgp3 and GlgA. The secretion system consisted of a segregated population of globular structures originating from midcycle reticulate bodies. Globular structures contained the Pgp4-regulated proteins CT143, CT144, and CT050 in addition to Pgp3 and GlgA. Genetic replacement of Pgp4 with Pgp3 or GlgA negated the formation of globular structures, resulting in retention of Pgp3 and GlgA in chlamydial organisms. The generation of globular structures and secretion of virulence factors occurred independently of type 2 and type 3 secretion systems. Globular structures were enriched with lipopolysaccharide but lacked detectable major outer membrane protein and heat shock protein 60,...Continue Reading

References

Mar 1, 1991·Microbiological Reviews·J W Moulder
Jul 1, 1986·Plasmid·L Palmer, S Falkow
Jun 20, 2003·Proceedings of the National Academy of Sciences of the United States of America·Robert J BellandHarlan D Caldwell
Sep 6, 2007·The Journal of Immunology : Official Journal of the American Association of Immunologists·Catherine M O'ConnellToni Darville
Jul 2, 2008·Infection and Immunity·Ine Jorgensen, Raphael H Valdivia
Oct 28, 2009·PLoS Neglected Tropical Diseases·Matthew J Burton, David C W Mabey
Mar 31, 2010·Infection and Immunity·Lacey D TaylorHarlan D Caldwell
May 25, 2011·Microbial Pathogenesis·Lei LeiGuangming Zhong
Sep 23, 2011·Chemical Reviews·Paige E SelvyH Alex Brown
Oct 12, 2011·The Journal of Experimental Medicine·Laszlo KariHarlan D Caldwell
Jun 25, 2013·Journal of Bacteriology·Siqi GongGuangming Zhong
Jul 31, 2013·PloS One·Chunxue LuGuangming Zhong
Feb 12, 2014·Methods in Molecular Biology·Efthalia ChronopoulouJody D Berry
Sep 17, 2015·Nature Reviews. Microbiology·Carmen Schwechheimer, Meta J Kuehn
Mar 17, 2016·ELife·Lena GehreAgathe Subtil
Apr 26, 2016·Nature Reviews. Microbiology·Cherilyn ElwellJoanne Engel
Jun 14, 2020·Pathogens and Disease·Chunmin HuYuanjun Liu
Jun 17, 2020·Journal of Bacteriology·Scot P OuelletteJohn V Cox

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