PMID: 1194353Nov 1, 1975Paper

A chlorophyll-protein complex lacking in photosystem I mutants of Chlamydomonas reinhardtii

The Journal of Cell Biology
N H ChuaP Bennoun

Abstract

Sodium dodecyl sulfate gel electrophoresis of unheated, detergent-solubilized thylakoid membranes of Chlamydomonas reinhardtii gives two chlorophyll-protein complexes. Chlorophyll-protein complex I (CP I) is the blue-green in color and can be dissociated by heat into "free" chlorophyll and a constituent polypeptide (polypeptide 2; mol wt 66,000). Similar experiments with spinach and Chinese cabbage show that the higher plant CP I contains an equivalent polypeptide but of slightly lower molecular weight (64,000). Both polypeptide 2 and its counterpart in spinach are soluble in a 2:1 (vol/vol) mixture of chloroform-methanol. Chemical analysis reveals that C. reinhardtii CP I has a chlorophyll a to b weight ratio of about 5 and that it contains approximately 5% of the total chlorophyll and 8-9% of the total protein of the thylakoid membranes. Thus, it can be calculated that each constituent polypeptide chain is associated with eight to nine chlorophyll molecules. Attempts to measure the molecular weight of CP I by calibrated SDS gels were unsuccessul since the complex migrates anomalously in such gels. Two Mendelian mutants of C. reinhardtii, F1 and F14, which lack P700 but have normal photosystem I activity, do not contain CP I o...Continue Reading

References

Jun 1, 1975·Proceedings of the National Academy of Sciences of the United States of America·N H Chua, P Bennoun
Apr 8, 1975·Biochimica Et Biophysica Acta·O Machold
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Jan 1, 1960·Proceedings of the National Academy of Sciences of the United States of America·N Sueoka

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Citations

Jun 12, 1996·Molecular & General Genetics : MGG·N FischerJ D Rochaix
Feb 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·G W SchmidtN H Chua
Mar 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·H S ShepherdN W Gillham
Jul 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·P Bennoun
May 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·G W Schmidt, M L Mishkind
Apr 1, 1985·The Journal of Cell Biology·M K Lyon, K R Miller
May 1, 1976·The Journal of Cell Biology·L Y Bourguignon, G E Palade
Jun 1, 1979·The Journal of Cell Biology·N H Chua, G W Schmidt
Feb 1, 1980·Plant Physiology·M Rusckowski, B A Zilinskas
Sep 1, 1980·European Journal of Biochemistry·B A Diner, F A Wollman
Aug 1, 1989·Molecular & General Genetics : MGG·E L de HostosA R Grossman
Jan 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·P Delepelaire, N H Chua
Nov 1, 1982·Archives of Biochemistry and Biophysics·Y TakahashiS Katoh

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