A choline-evoked [Ca2+]c signal causes catecholamine release and hyperpolarization of chromaffin cells

FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology
Jorge FuentealbaAntonio G García

Abstract

In bovine chromaffin cells fast-superfused with Krebs-HEPES solution containing 1-2 mM Ca2+, 5 s pulses of choline (1-10 mM), elicited catecholamine secretory responses that were only approximately 10% of those evoked by ACh (0.01-0.1 mM). However, in high-Ca2+ solutions (10-20 mM) the size of the choline secretory responses approached those of ACh. The choline responses (10 mM choline in 20 mM Ca2+, 10Cho/20Ca2+) tended to decline upon repetitive pulsing, whereas those of ACh were well maintained. The confocal [Ca2+]c increases evoked by 10Cho/20Ca2+ were similar to those of ACh. Whereas 10Cho/20Ca2+ caused mostly hyperpolarization of chromaffin cells, 0.1ACh/20 Ca2+ caused first depolarization and then hyperpolarization; in regular solutions (2 mM Ca2+), the hyperpolarizing responses did not show up. In Xenopus oocytes injected with mRNA for bovine alpha7 nicotinic receptors (nAChRs), 10Cho/20 Ca2+ fully activated an inward current; in oocytes expressing alpha3beta4, however, the inward current elicited by choline amounted to only 4% of the size of alpha7 current. Our results suggest that choline activates the entry of Ca2+ through alpha7 nAChRs; this leads to a cytosolic concentration of calcium ([Ca2+]c) rise that causes th...Continue Reading

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Citations

Nov 28, 2012·Brain Research Bulletin·Balázs LendvaiZsolt Némethy
Mar 15, 2006·European Journal of Pharmacology·Juana Maria González-RubioJesús M Hernández-Guijo
Mar 21, 2006·European Journal of Pharmacology·Juana M González-RubioLuis Gandía
Jul 13, 2007·Neurochemistry International·Szilard I SzaboBalazs Lendvai
Apr 9, 2008·Physiological Reviews·Balázs Lendvai, E Sylvester Vizi
Feb 13, 2009·American Journal of Physiology. Cell Physiology·Laura TapiaLuis Gandía
Jul 15, 2018·The Journal of Pharmacology and Experimental Therapeutics·Carmen NanclaresAntonio G García
Jul 25, 2009·British Journal of Pharmacology·R L JonesD F Woodward
Apr 9, 2021·Frontiers in Behavioral Neuroscience·Yasmine SherafatChristie D Fowler

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