A chromogenic assay for limit dextrinase and pullulanase activity

Analytical Biochemistry
Marie BøjstrupOle Hindsgaul

Abstract

A new chromogenic substrate to assay the starch debranching enzymes limit dextrinase and pullulanase is described. The 2-chloro-4-nitrophenyl glycoside of a commercially available branched heptasaccharide (Glc-maltotriosyl-maltotriose) was found to be a suitable specific substrate for starch debranching enzymes and allows convenient assays of enzymatic activities in a format suited for high-throughput analysis. The kinetic parameters of these enzymes toward the synthesized substrate are determined, and the selectivity of the substrate in a complex cereal-based extract is established.

References

Jul 1, 1984·Journal of Clinical Chemistry and Clinical Biochemistry. Zeitschrift Für Klinische Chemie Und Klinische Biochemie·E HenkelR Henkel
Sep 8, 2009·Protein Expression and Purification·Malene Bech Vester-ChristensenBirte Svensson

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Citations

Dec 1, 2015·Journal of Agricultural and Food Chemistry·Marie BøjstrupOle Hindsgaul
Feb 7, 2021·World Journal of Microbiology & Biotechnology·Pei XuWen-Yong Lou

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