PMID: 9634770Mar 1, 1995Paper

A chromosome integration system for stable gene transfer into Thermus flavus

Bio/technology
J M WeberD C Demirjian

Abstract

We have developed a chromosomal integration system for gene transfer into the extreme thermophile Thermus flavus. The system relies on integration at the site of leuB (3-isopropylmalate dehydrogenase) which was cloned from T. flavus. The leuB gene was insertionally inactivated in vitro with a thermostable kanamycin-resistance gene and transformed in single-copy into the chromosome of T. flavus on a plasmid vector. Gene replacement strains required leucine for growth, were stably kanamycin-resistant and could grow in the presence of kanamycin at temperatures up to 55 degrees C.

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Citations

Sep 8, 2004·Applied Microbiology and Biotechnology·S H BjornsdottirG Eggertsson
Jun 28, 2011·Applied and Environmental Microbiology·Snaedis H BjornsdottirGudmundur Eggertsson
Aug 30, 2001·Applied and Environmental Microbiology·O Fridjonsson, R Mattes
Jun 1, 2010·Journal of Bacteriology
Mar 16, 2005·Protein Expression and Purification·Ying ChenJames A Fee

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