A circulating tumor cell-based digital assay for the detection of EGFR T790M mutation in advanced non-small cell lung cancer.

Journal of Materials Chemistry. B, Materials for Biology and Medicine
Jing WangHongguang Zhu

Abstract

Determining the status of epidermal growth factor receptor (EGFR) T790M mutation is crucial for guiding further treatment intervention in advanced non-small cell lung cancer (NSCLC) patients who develop acquired resistance to initial EGFR tyrosine kinase inhibitor (TKI) treatment. Circulating tumor cells (CTCs) which contain plentiful copies of well-preserved RNA offer an ideal source for noninvasive detection of T790M mutation in NSCLC. We developed a CTC-based digital assay which synergistically integrates NanoVelcro Chips for enriching NSCLC CTCs and reverse-transcription droplet digital PCR (RT-ddPCR) for quantifying T790M transcripts in the enriched CTCs. We collected 46 peripheral arterial and venous blood samples from 27 advanced NSCLC patients for testing this CTC-based digital assay. The results showed that the T790M mutational status observed by the CTC-based digital assay matched with those observed by tissue-based diagnostic methods. Furthermore, higher copy numbers of T790M transcripts were observed in peripheral arterial blood than those detected in the matched peripheral venous blood. In short, our results demonstrated the potential of the NanoVelcro CTC-digital assay for noninvasive detection of the T790M mutati...Continue Reading

References

Jul 14, 2004·Proceedings of the National Academy of Sciences of the United States of America·Robert T KrivacicRichard H Bruce
Aug 20, 2004·The New England Journal of Medicine·Massimo CristofanilliDaniel F Hayes
Feb 25, 2005·The New England Journal of Medicine·Susumu KobayashiBalázs Halmos
Nov 7, 2006·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Marissa N BalakWilliam Pao
Apr 4, 2007·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·David R ShafferHoward I Scher
Jul 3, 2007·Proceedings of the National Academy of Sciences of the United States of America·Wei HePhilip S Low
Oct 12, 2010·Proceedings of the National Academy of Sciences of the United States of America·Shannon L StottMehmet Toner
Oct 23, 2010·Nature Reviews. Cancer·William Pao, Juliann Chmielecki
May 13, 2011·Nature Reviews. Cancer·Heidi SchwarzenbachKlaus Pantel
Aug 2, 2011·Lab on a Chip·Albert J MachDino Di Carlo
Jul 24, 2012·Nature Biotechnology·Daniel RamsköldRickard Sandberg
Apr 5, 2013·Science Translational Medicine·Emre OzkumurMehmet Toner
Jul 10, 2013·Nature Reviews. Clinical Oncology·Emily CrowleyAlberto Bardelli
Jan 15, 2014·Journal of Thoracic Oncology : Official Publication of the International Association for the Study of Lung Cancer·Yuankai ShiPan-Chyr Yang
Jan 22, 2014·Nature Reviews. Clinical Oncology·Matthew G KrebsCaroline Dive
Jan 23, 2014·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·Luis A Diaz, Alberto Bardelli
Jul 25, 2014·Nature Reviews. Cancer·Zhao ChenKwok-Kin Wong
Aug 12, 2014·Accounts of Chemical Research·Millicent LinHsian-Rong Tseng
Jul 17, 2015·JAMA Oncology·Niki KarachaliouUNKNOWN Spanish Lung Cancer Group
Jan 10, 2016·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Chris KarlovichHeather Wakelee
Jun 30, 2016·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·Geoffrey R OxnardPasi A Jänne
Jul 30, 2016·Journal of Thoracic Oncology : Official Publication of the International Association for the Study of Lung Cancer·Phil A J CrosbieCaroline Dive
Aug 18, 2016·Clinical Lung Cancer·Tasneem LokhandwalaDavid P Carbone
Oct 7, 2016·Advanced Science·Rhonda M JackSunitha Nagrath
Dec 14, 2016·The New England Journal of Medicine·Tony S MokUNKNOWN AURA3 Investigators
Jan 18, 2017·Proceedings of the National Academy of Sciences of the United States of America·Mark KalinichDaniel A Haber
Apr 11, 2017·Chemical Society Reviews·Zheyu ShenXiaoyuan Chen
Feb 18, 2018·Proceedings of the National Academy of Sciences of the United States of America·Xin HongDaniel A Haber
Mar 15, 2018·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Elena Castellanos-RizaldosJohan K Skog
Mar 20, 2018·Advanced Drug Delivery Reviews·Yu Jen JanHsian-Rong Tseng
Apr 7, 2018·Liver Transplantation : Official Publication of the American Association for the Study of Liver Diseases and the International Liver Transplantation Society·Colin M CourtVatche G Agopian
Oct 9, 2019·Nature Medicine·Francesca ChemiUNKNOWN TRACERx Consortium

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Citations

Nov 27, 2020·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Qi WangChun Mao

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Methods Mentioned

BETA
biopsy
Assay
Chips
PCR
blood draw
biopsies
chip

Software Mentioned

NanoVelcro
QuantaSoft
GraphPad Prism

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