A cis-regulatory module activating transcription in the suspensor contains five cis-regulatory elements

Plant Molecular Biology
Kelli F HenryRobert B Goldberg

Abstract

Little is known about the molecular mechanisms by which the embryo proper and suspensor of plant embryos activate specific gene sets shortly after fertilization. We analyzed the upstream region of the Scarlet Runner Bean (Phaseolus coccineus) G564 gene in order to understand how genes are activated specifically in the suspensor during early embryo development. Previously, we showed that a 54-bp fragment of the G564 upstream region is sufficient for suspensor transcription and contains at least three required cis-regulatory sequences, including the 10-bp motif (5'-GAAAAGCGAA-3'), the 10 bp-like motif (5'-GAAAAACGAA-3'), and Region 2 motif (partial sequence 5'-TTGGT-3'). Here, we use site-directed mutagenesis experiments in transgenic tobacco globular-stage embryos to identify two additional cis-regulatory elements within the 54-bp cis-regulatory module that are required for G564 suspensor transcription: the Fifth motif (5'-GAGTTA-3') and a third 10-bp-related sequence (5'-GAAAACCACA-3'). Further deletion of the 54-bp fragment revealed that a 47-bp fragment containing the five motifs (the 10-bp, 10-bp-like, 10-bp-related, Region 2 and Fifth motifs) is sufficient for suspensor transcription, and represents a cis-regulatory module....Continue Reading

References

Feb 1, 1997·Trends in Genetics : TIG·C Martin, J Paz-Ares
Dec 10, 1998·Nucleic Acids Research·K HigoT Korenaga
Nov 10, 2001·The Plant Cell·K WeteringsR B Goldberg
Oct 1, 1993·The Plant Cell·E. C. Yeung, D. W. Meinke
Jan 10, 2003·Nucleic Acids Research·V MatysE Wingender
Apr 3, 2003·Genome Research·Alexander P LifanovDmitri A Papatsenko
Jun 3, 2004·Genome Research·Gavin E CrooksSteven E Brenner
Jun 9, 2007·Plant Physiology·Brandon H LeRobert B Goldberg
Oct 28, 1994·Science·R B GoldbergR Yadegari
Feb 12, 2009·Proceedings of the National Academy of Sciences of the United States of America·Tomokazu KawashimaRobert B Goldberg
Sep 22, 2009·Developmental Biology·R Andrew Cameron, Eric H Davidson
Dec 8, 2009·Trends in Plant Science·Tomokazu Kawashima, Robert B Goldberg
Aug 8, 2012·Nature Reviews. Genetics·François Spitz, Eileen E M Furlong
Jan 16, 2013·Proceedings of the National Academy of Sciences of the United States of America·Mark F BelmonteJohn J Harada
Jun 9, 2014·Nature Genetics·Jeremy SchmutzScott A Jackson
Feb 24, 2015·Frontiers in Plant Science·Kelli F Henry, Robert B Goldberg

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Citations

Dec 7, 2019·Physiologia Plantarum·Downs Jacob, Jones Brian
Jun 6, 2018·Proceedings of the National Academy of Sciences of the United States of America·Kelli F HenryRobert B Goldberg
Aug 23, 2018·Plant Cell Reports·Agnieszka ChmielnickaJustyna Teresa Polit

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Datasets Mentioned

BETA
GSE57537

Methods Mentioned

BETA
transgenic
PCR
Illumina
RNA-Seq
one-hybrid

Software Mentioned

Scarlet Runner Bean
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