A citrate-inducible gene, encoding a putative tricarboxylate transporter, is downregulated by the organic solvent DMSO in Agrobacterium tumefaciens

Journal of Applied Microbiology
A-C LiuE M Lai

Abstract

To investigate the effects of the organic solvent dimethyl sulfoxide (DMSO) on the expression of a citrate-inducible gene, encoding a putative tricarboxylate transporter, in Agrobacterium tumefaciens. By two-dimensional gel electrophoresis, we discovered a putative tricarboxylate transporter named ActC, whose expression was downregulated by DMSO. The expression of actC is also induced by tricarboxylates but not affected by other organic acids of the TCA cycle. Intriguingly, transcriptional activation of actC by citrate is compromised in the presence of DMSO. Furthermore, expression of actC is abolished by deletion of actDE, encoding a putative two-component regulatory system upstream of the actCBA gene cluster. actC is a citrate-inducible gene that is repressed by DMSO and whose expression is likely regulated by a two-component system. This study provides useful information as to a potential DMSO-regulatory system of A. tumefaciens or other soil bacteria when encountering DMSO in nature. In addition, DMSO-regulated genes should be taken into account for studies in which bacterial cultures were treated with compounds dissolved in DMSO.

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Citations

Mar 7, 2012·The Journal of Biological Chemistry·Lay-Sun MaErh-Min Lai
Aug 2, 2011·Journal of Bacteriology·Meriyem AktasFranz Narberhaus
Jun 18, 2016·Proceedings of the National Academy of Sciences of the United States of America·Devanand D BondageErh-Min Lai

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