A classical enhancer element responsive to both lipopolysaccharide and interferon-gamma augments induction of the iNOS gene in mouse macrophages

Gene
E W AlleyS W Russell

Abstract

The ability of macrophages to kill some kinds of tumor cells is dependent upon the production of the free radical nitric oxide (NO) by the inducible enzyme NO synthase (iNOS; EC 1.14.13.39). Expression of the iNOS gene is induced by lipopolysaccharide (LPS) and augmented by interferon-gamma (IFN-gamma). Two regions of the iNOS promoter are known to regulate induction, a promoter proximal region I (RI) and a more distal region II (RII). Reconfiguration of RI within the iNOS regulatory region revealed its dependence upon native position and orientation for maximal activity, suggesting that it is a core promoter module, and further implicated the putative octamer element as a contributor to promoter activity. RII, however, functioned in a relatively orientation- and position-independent manner. Therefore, it had the characteristics of a classical enhancer element.

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Citations

Apr 11, 2008·The Journal of Biological Chemistry·Ashwani KumarAdrie J C Steyn
Dec 16, 1998·Nitric Oxide : Biology and Chemistry·A MizutaniN Tsukagoshi
Oct 24, 2006·Biochemical and Biophysical Research Communications·Hee-Sae ParkEui-Ju Choi
Apr 7, 2017·Scientific Reports·Kirstin BilhamAdrian L Smith
Sep 20, 2005·The Journal of Biological Chemistry·Mark W FeinbergMukesh K Jain
Sep 19, 2008·Journal of Leukocyte Biology·Nuria RodriguezThomas Miethke
Jul 17, 2010·The Journal of Biological Chemistry·Jidnyasa GaekwadGeert-Jan Boons
Sep 10, 2004·The Journal of Immunology : Official Journal of the American Association of Immunologists·Rebecca M BaronMark A Perrella
Jan 24, 2014·Cancer Research·Weifang LingYufang Shi
Sep 23, 2006·Journal of Pharmacological and Toxicological Methods·Saule NaureckienePhilip G Jones

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