A clinically applicable method for determining the three major alleles at the Duffy (FY) blood group locus using polymerase chain reaction with allele-specific primers

Transfusion
M L OlssonGeoff Daniels

Abstract

The clinically significant antigens of the Duffy (Fy [FY]) blood group system are expressed on the red cell form of the FY glycoprotein, a promiscuous chemokine receptor and also a receptor for malarial parasites. After the cloning of cDNA coding for FY glycoprotein, the molecular basis of the three major alleles (Fya/Fyb/Fy) has been established. Because of the mistyping of the silent Fy allele as Fyb, the error rate of current genotyping methods is high in black populations. Two hundred blood donors (European whites and African Blacks) and some amniotic DNA samples were investigated by a new allele-specific primer polymerase chain reaction technique. Sense primers corresponding to normal and GATA-1-mutated FY gene promoter region sequences were combined with antisense primers discriminating the Fya/Fyb polymorphism. Complete correlation between FY phenotypes and genotypes was obtained in all samples studied, although, in two whites and one black, serology showed weak Fyb expression while polymerase chain reaction indicated a Fyb allele. Gene frequencies were calculated. This simple and rapid polymerase chain reaction method was shown to detect the three common alleles at the FY locus in two representative ethnic populations. ...Continue Reading

Citations

Jul 3, 2010·Journal of Immigrant and Minority Health·Joshua K ElsonAlex B Lentsch
Nov 6, 2001·Clinica Chimica Acta; International Journal of Clinical Chemistry·F Y Lau, G Cheng
Sep 12, 1998·British Journal of Haematology·N M IrshaidM L Olsson
Jul 14, 2001·Transfusion·L YanL Li
Sep 19, 2002·American Journal of Therapeutics·Marc Germain, Mindy Goldman
Feb 15, 2002·Current Opinion in Hematology·Marion E Reid, Christine Lomas-Francis
Aug 15, 1998·Vox Sanguinis·N D Avent
Apr 19, 2012·Malaria Journal·Agnieszka Kempińska-PodhorodeckaAndrzej Ciechanowicz
Sep 12, 2008·Malaria Journal·Richard L CulletonKazuyuki Tanabe
Dec 31, 2011·Chest·Kirsten Neudoerffer KangelarisUNKNOWN National Heart, Lung, and Blood Institute ARDS Network
Mar 15, 2014·Memórias do Instituto Oswaldo Cruz·Daniela Camargos CostaLuzia Helena Carvalho
Apr 30, 2015·Transfusion and Apheresis Science : Official Journal of the World Apheresis Association : Official Journal of the European Society for Haemapheresis·Silvia ManfroiPasqualepaolo Pagliaro
May 22, 2007·Transfusion clinique et biologique : journal de la Société française de transfusion sanguine·Neil D Avent
Dec 1, 2015·Transfusion and Apheresis Science : Official Journal of the World Apheresis Association : Official Journal of the European Society for Haemapheresis·Alhassane BaMonique Silvy
Jun 18, 2004·Vox Sanguinis·G Daniels
Jun 24, 2004·Vox Sanguinis·M L Olsson
Jun 24, 2004·Vox Sanguinis·C E van der Schoot
Dec 1, 2004·Vox Sanguinis·L CastilhoM E Reid
Jul 19, 2003·Transfusion·Maryse St-LouisRéal Lemieux
Jul 31, 2007·Transfusion·Jill R StorryMarion E Reid
Sep 12, 2015·Annals of Laboratory Medicine·Oytip NathalangPawinee Kupatawintu
May 7, 2014·Journal of Clinical Laboratory Analysis·Shihang ZhouFengyuan Piao
Dec 3, 2013·Parasitology International·Tetsuya FuruyaTimothy T Stedman
Oct 2, 2013·Methods : a Companion to Methods in Enzymology·S A BoccozC A Marquette
Jan 29, 2002·Revista Da Sociedade Brasileira De Medicina Tropical·C E CavasiniM U Ferreira
Dec 23, 2003·American Journal of Human Biology : the Official Journal of the Human Biology Council·Frédéric BauduerJacques Chiaroni
Jan 9, 1999·Transfusion Medicine·W A FlegelC Gassner
Dec 7, 2018·Genetics and Molecular Biology·Haiala S Silva de OliveiraJoão Farias Guerreiro

❮ Previous
Next ❯

Related Concepts

Related Feeds

Blood Group Incompatibility

Blood group incompatibility between donor and recipient has been associated with poor transplant outcomes. Discover the latest research on blood group icompatibility here.