A cloned DNA fragment for identification of Mycobacterium tuberculosis

Reviews of Infectious Diseases
R PatelD F Wirth

Abstract

The identification of Mycobacterium tuberculosis is a lengthy process. Attempts were made to develop a more rapid, specific, and sensitive assay for identifying tubercle bacilli in biologic specimens by differentially screening plasmid libraries of Sau3AI-digested M. tuberculosis H37Rv (ATCC 25618) DNA with homologous and heterologous DNA for clones that hybridized strongly with M. tuberculosis DNA alone. Three clones, pMTB1, pMTb2, and pMTb3, were selected for further study. Southern analysis indicated that these clones reacted strongly with DNA from strains of M. tuberculosis isolated in different parts of the world, weakly with DNA from other mycobacterial species, and not at all with Escherichia coli or human DNA. Smaller fragments of mycobacterial DNA contained in plasmid pMTb3 were subcloned into pBR322 (pMTb4) or pUC12 (pMTb5). These recombinant plasmids hybridized with DNA from M. tuberculosis, Mycobacterium bovis, and Mycobacterium bovis BCG (bacille Calmette-Guérin) Montreal and may provide the reagents needed for the development of new methods for rapid diagnosis of M. tuberculosis infections.

Citations

Feb 1, 1995·Veterinary Microbiology·B J WardsG W de Lisle
Oct 1, 1992·Tubercle and Lung Disease : the Official Journal of the International Union Against Tuberculosis and Lung Disease·D de WitL Steyn
Aug 1, 1991·Journal of Clinical Microbiology·J W FriesD F Wirth
Nov 1, 1990·Journal of Clinical Microbiology·D De WitM Sogin
Jan 1, 1993·The American Journal of the Medical Sciences·J C Weissler

❮ Previous
Next ❯

Related Concepts

Related Feeds

Allergy & Infectious Diseases

Allergies result from the hyperreactivity of the immune system to some environmental substance and can be life-threatening. Infectious diseases are caused by organisms including bacteria, viruses, fungi and parasites. They can be transmitted different ways, such as person-to-person. Here is the latest research on allergy and infectious diseases.