A cloned major Schistosoma mansoni egg antigen with homologies to small heat shock proteins elicits Th1 responsiveness.

Infection and Immunity
Y CaiD L Boros

Abstract

In schistosomiasis mansoni, soluble egg antigens of the worm induce chronic T-cell-mediated granulomatous tissue responses. Since the first preparation of crude soluble egg antigen extract, a dearth of highly purified antigens has hampered the identification of granuloma inducer molecules. Here we report that a cloned 38-kDa egg polypeptide (r38) with homologies to small heat shock proteins is a strong immunogen. The recombinant and the sodium dodecyl sulfate-polyacrylamide gel electrophoresis separated and eluted native 38-kDa (p38) polypeptides, used in microgram amounts and unaided by adjuvant, sensitized mice for a Th1-type immune response, with strong interleukin-2 (IL-2) and gamma interferon secretion but no IL-4 and IL-10 secretion. Extensive cross-reactivity between these two polypeptides was evident. THis pattern was confirmed by reverse transcription-PCR that showed strong IL-2 and gamma interferon message expression but trace amounts of IL-4 message expression in r38-sensitized splenocytes. In mice, the polypeptide induced pulmonary mononuclear granuloma formation around antigen-coupled beads or worm eggs. We propose that the superior immunogenicity of r38 is linked to its relatedness to small heat shock proteins and...Continue Reading

References

Jan 1, 1992·Memórias do Instituto Oswaldo Cruz·D L Boros, N W Lukacs
Apr 1, 1992·Molecular and Biochemical Parasitology·D MoserM Q Klinkert
Oct 1, 1991·European Journal of Immunology·A R LussowG Del Giudice
Sep 1, 1991·The Journal of Experimental Medicine·J B HaanenH Spits
Mar 1, 1991·Immunology Today·B S Polla
Jul 1, 1989·Clinical Microbiology Reviews·D L Boros
Jan 2, 1987·Molecular and Biochemical Parasitology·K S JohnsonJ S Cordingley
Apr 1, 1974·Experimental Parasitology·L M HangD L Boros
Jan 1, 1981·Transactions of the Royal Society of Tropical Medicine and Hygiene·D W DunneM J Doenhoff

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Citations

Sep 14, 2004·Immunological Reviews·Miguel J StadeckerJing Sun
Oct 26, 2012·Seminars in Immunopathology·Bridget M LarkinMiguel J Stadecker
Jun 6, 2015·Cell Stress & Chaperones·Deyanira Pérez-Morales, Bertha Espinoza
Mar 7, 2020·Frontiers in Immunology·Bing ZhengChaohong Liu
May 9, 2000·The Journal of Immunology : Official Journal of the American Association of Immunologists·I O FarahC L King
Nov 9, 2005·European Journal of Immunology·Laura E LaylandClarissa U Prazeres da Costa
Feb 11, 2014·Current Protocols in Immunology·Matthew S TuckerYung-San Liang

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