A closer look at Verogen's Forenseq™ DNA Signature Prep kit autosomal and Y-STR data for streamlined analysis of routine reference samples

Electrophoresis
Lilliana I MorenoRebecca S Just

Abstract

Massively parallel sequencing (MPS) provides forensic DNA laboratories an option to overcome the limitations associated with CE and current STR assays. Verogen's MPS ForenseqTM DNA Signature kit concomitantly amplifies 27 autosomal, 7 X-, and 24 Y-STRs. In addition, 94 identity, 56 ancestry, and 22 phenotypic-informative SNPs are included for a total of over 200 markers in one multiplex. An internal validation of this platform was conducted using reference samples to investigate whether the Forenseq™ DNA Signature Prep kit, specifically primer panel B, has the capability to provide consistent and accurate typing/sequencing data. The data presented in this report is limited to that corresponding to autosomal and Y-STRs. Results suggest that the system can consistently generate accurate genotyping data when up to 40 high-quality, high-quantity (i.e. 1 ng) single source samples are pooled into a sequencing reaction. The results generated were used to determine appropriate analysis parameters and thresholds for streamlined data interpretation of reference samples.

References

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Citations

Nov 21, 2019·International Journal of Legal Medicine·Petra MüllerUNKNOWN SeqForSTR-Consortium
Dec 12, 2018·BMJ : British Medical Journal·Gal KoplewitzAnupam B Jena
Jan 2, 2021·Forensic Science International : Synergy·John M Butler, Sheila Willis
Jan 26, 2021·Forensic Science International. Genetics·Jonathan L KingBruce Budowle
Oct 20, 2019·Forensic Science International. Genetics·Michael D BrandhagenJodi A Irwin
Apr 2, 2021·Emerging Topics in Life Sciences·Penelope R Haddrill
Jun 21, 2020·Forensic Science International. Genetics·Øyvind BlekaPeter Gill
Nov 30, 2018·Analytical Chemistry·Bruce R McCordGeorge Duncan

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