Abstract
Plasmid pMET7C containing a 6.05 kb DNA insert from Clostridium acetobutylicum P262 made Escherichia coli F19 cells sensitive to metronidazole. The nucleotide sequence of the C. acetobutylicum DNA controlling metronidazole sensitivity in E. coli F19 revealed an ORF of 972 bp which encoded a protein of 324 amino acids with a calculated Mr of 35,000. The amino acid sequence encoded by the ORF contained a helix-turn-helix DNA-binding domain and was homologous to the catabolite control protein, CcpA, from Bacillus subtilis and Bacillus megaterium, a tRNA repressor of E. coli encoded by the shl gene, and the GalR, Lacl and PurR repressors of E. coli. The C. acetobutylicum ORF, which was termed regA, complemented a B. subtilis ccpA mutant and an E. coli shl mutant, but was unable to complement E. coli galR, lacl or purR mutants. To determine whether the regA gene product was involved in the regulation of amylase gene expression in C. acetobutylicum, a starch-degrading enzyme gene (staA) from C. acetobutylicum NCIMB 8052 was cloned. The RegA protein inhibited the degradation of starch by the C. acetobutylicum staA gene product in E. coli.
Citations
Jan 22, 2005·Applied Microbiology and Biotechnology·Sharon J Reid, Valerie R Abratt
Oct 1, 1995·FEMS Microbiology Reviews·D R Woods, S J Reid
Jun 1, 1996·FEMS Microbiology Letters·E KüsterW Hillen
Jul 1, 1996·Research in Microbiology·S Chauvaux
Oct 12, 2001·Toxicon : Official Journal of the International Society on Toxinology·O Matsushita, A Okabe
Mar 27, 1999·Molecular Microbiology·E J LuesinkW M de Vos
Feb 13, 1999·Molecular Microbiology·A KrausW Hillen
Jan 5, 2000·Applied and Environmental Microbiology·K MahrF Titgemeyer
Aug 19, 2003·Journal of Bacteriology·Tali W DrorYuval Shoham
Aug 5, 2004·Journal of Bacteriology·John VargaStephen B Melville
Dec 17, 2004·Journal of Bacteriology·Yi-Heng Percival Zhang, Lee R Lynd
Dec 13, 2006·Microbiology and Molecular Biology Reviews : MMBR·Josef DeutscherPieter W Postma
Aug 5, 1998·Proceedings of the National Academy of Sciences of the United States of America·J H KimG H Chambliss
May 12, 2012·Journal of Biomedicine & Biotechnology·Li-Wei LeeSzecheng J Lo
Dec 18, 1995·FEBS Letters·C C Nguyen, M H Saier
Jun 29, 2001·Microbiology·S KeisD T Jones
Feb 13, 2001·Microbiology·S BehrensH Bahl
Jan 1, 1996·FEMS Microbiology Letters·T M Henkin
Jun 17, 2016·Biotechnology and Applied Biochemistry·Shuai MaiGehua Wang
Dec 22, 2006·Journal of Molecular Microbiology and Biotechnology·Wilfrid J MitchellPhilip G Meaden
Mar 3, 2004·Microbiology·Carlos D EstebanFritz Titgemeyer
May 21, 2020·Plant & Cell Physiology·Motoki TanakaYukako Hihara
May 9, 1998·Infection and Immunity·C L Simpson, R R Russell
Dec 29, 1998·Journal of Bacteriology·R V NairE T Papoutsakis
Dec 22, 1999·Journal of Bacteriology·E Küster-SchöckW Hillen
Nov 13, 1998·Journal of Bacteriology·A J TurinskyT M Henkin
Jan 1, 1996·Journal of Bacteriology·M Gering, R Brückner
Oct 23, 1997·Journal of Bacteriology·J NguyenM Guérineau
Nov 14, 1997·Journal of Bacteriology·V MonederoG Pérez-Martínez