A Clostridium difficile-Specific, Gel-Forming Protein Required for Optimal Spore Germination

MBio
M Lauren DonnellyAimee Shen

Abstract

Clostridium difficile is a Gram-positive spore-forming obligate anaerobe that is a leading cause of antibiotic-associated diarrhea worldwide. In order for C. difficile to initiate infection, its aerotolerant spore form must germinate in the gut of mammalian hosts. While almost all spore-forming organisms use transmembrane germinant receptors to trigger germination, C. difficile uses the pseudoprotease CspC to sense bile salt germinants. CspC activates the related subtilisin-like protease CspB, which then proteolytically activates the cortex hydrolase SleC. Activated SleC degrades the protective spore cortex layer, a step that is essential for germination to proceed. Since CspC incorporation into spores also depends on CspA, a related pseudoprotease domain, Csp family proteins play a critical role in germination. However, how Csps are incorporated into spores remains unknown. In this study, we demonstrate that incorporation of the CspC, CspB, and CspA germination regulators into spores depends on CD0311 (renamed GerG), a previously uncharacterized hypothetical protein. The reduced levels of Csps in gerG spores correlate with reduced responsiveness to bile salt germinants and increased germination heterogeneity in single-spore ge...Continue Reading

References

Feb 13, 2002·Current Opinion in Structural Biology·H Jane Dyson, Peter E Wright
May 23, 2002·Biochemistry·A Keith DunkerZoran Obradović
Apr 3, 2003·Journal of Bacteriology·Rosa-Martha Cabrera-MartinezPeter Setlow
Dec 10, 2003·Current Opinion in Microbiology·Peter Setlow
Feb 5, 2008·Journal of Bacteriology·Joseph A Sorg, Abraham L Sonenshein
Feb 24, 2009·Current Protocols in Microbiology·Joseph A Sorg, Sean S Dineen
May 19, 2009·Journal of Microbiological Methods·John T HeapNigel P Minton
Oct 7, 2009·Proceedings of the National Academy of Sciences of the United States of America·Christian OttmannAndreas Schaller
Nov 7, 2009·Journal of Microbiological Methods·John T HeapNigel P Minton
Dec 22, 2009·Applied and Environmental Microbiology·Stephen T Cartman, Nigel P Minton
Jan 27, 2010·Biochimica Et Biophysica Acta·Bin XueVladimir N Uversky
Nov 30, 2010·Trends in Microbiology·Daniel Paredes-SabjaMahfuzur R Sarker
Jan 12, 2011·Nature Biotechnology·James T RobinsonJill P Mesirov
Mar 8, 2011·Applied Microbiology and Biotechnology·Hue Nguyen Thi MinhPatrick Gervais
Sep 29, 2011·Journal of Bacteriology·Patima PermpoonpattanaSimon M Cutting
Nov 24, 2011·The American Journal of Gastroenterology·Sahil KhannaAlan R Zinsmeister
Feb 14, 2012·Applied and Environmental Microbiology·Arturo Ramirez-PeraltaPeter Setlow
May 23, 2012·Infection and Immunity·Laura J DeakinTrevor D Lawley
Aug 21, 2012·Journal of Bacteriology·Xuan Yi ButzinPeter Setlow
Jan 8, 2013·Journal of Bacteriology·Emily E PutnamAimee Shen
Jul 10, 2013·Environmental Microbiology·Natalya Yutin, Michael Y Galperin
Aug 21, 2013·PLoS Genetics·Kelly A FimlaidAimee Shen
Aug 31, 2013·PLoS Pathogens·Vasant Muralidharan, Daniel E Goldberg
Oct 8, 2013·PLoS Genetics·Fátima C PereiraAdriano O Henriques
Feb 4, 2014·Journal of Bacteriology·Peter Setlow

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Citations

Feb 28, 2018·Proteomics. Clinical Applications·Bhagyashree Nandakishor SwargeLeo J de Koning
Feb 24, 2018·Frontiers in Cellular and Infection Microbiology·Duolong ZhuXingmin Sun
Sep 7, 2017·Journal of Bacteriology·Yuzo Kevorkian, Aimee Shen
Aug 15, 2018·Journal of Applied Microbiology·Peter Setlow
May 11, 2017·The Journal of Biological Chemistry·Ritu ShresthaJoseph A Sorg
Jan 30, 2019·Journal of Bacteriology·Megan H TouchetteAimee Shen
Jul 13, 2017·Annual Review of Microbiology·Peter SetlowYong-Qing Li
May 16, 2018·Journal of Bacteriology·Travis J KochanPhilip C Hanna
Apr 30, 2019·The Journal of the American Osteopathic Association·Robert OrensteinM Teresa Seville
Aug 21, 2020·Journal of Bacteriology·Hector Benito de la PueblaAimee Shen
Dec 21, 2019·Microbiology Spectrum·Aimee ShenDaniel Paredes-Sabja

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Methods Mentioned

BETA
RNA-Seq

Software Mentioned

PLAAC ( A mino A cid C omposition [
Integrative Genomics Viewer
TargeTron

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