A cluster of alpha 2-macroglobulin-related genes (alpha 2 M) on human chromosome 12p: cloning of the pregnancy-zone protein gene and an alpha 2M pseudogene.

Gene
K DevriendtP Marynen

Abstract

The characterization of two alpha 2-macroglobulin (alpha 2M)-related genomic clones, isolated from two human genomic libraries by use of alpha 2M cDNA [Kan et al., Proc. Natl. Acad. Sci. USA 82 (1985) 2282-2286] as a probe, is reported. Sequence comparison of the clone EPZP6 with the human alpha 2M cDNA revealed the presence of five exons with the proper splice signals. Alignment of the corresponding amino acid (aa) sequence of these exons with the published partial pregnancy-zone protein (PZP) aa sequence (Sottrup-Jensen et al., Proc. Natl. Acad. Sci. USA 81 (1984) 7353-7357] showed a perfect match, thereby identifying EPZP6 as a PZP genomic clone. The clone MPAM16 showed a considerable degree of sequence conservation when compared to the human alpha 2M cDNA sequence, and several putative exons were identified. However, a frame-shift mutation leading to a premature stop codon was found in the coding sequence, classifying this gene as an alpha 2M pseudogene. Human alpha 2M, PZP and the related pseudogene were mapped to the human chromosome 12p12-13, with the help of gene-specific probes and in situ hybridization. This result was confirmed in Southern-blot experiments with DNA from a human-Ltk- mouse somatic-cell hybrid containi...Continue Reading

References

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Jan 1, 1978·The International Journal of Biochemistry·W H Stimson, D M Farquharson
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Jul 15, 1974·American Journal of Obstetrics and Gynecology·B von Schoultz
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Jul 26, 1982·Biochimica Et Biophysica Acta·D E Panrucker, F L Lorscheider
Dec 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·L Sottrup-JensenB F Tack
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Mar 5, 1981·Clinica Chimica Acta; International Journal of Clinical Chemistry·J FolkersenP Hindersson

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Citations

Jan 1, 1992·Mammalian Genome : Official Journal of the International Mammalian Genome Society·C HillikerH Van den Berghe
Apr 1, 1990·Cancer Genetics and Cytogenetics·J ZhangJ J Cassiman
Jun 26, 2003·The International Journal of Biochemistry & Cell Biology·Yan LingNoor Kalsheker
Apr 30, 1992·Biochemical and Biophysical Research Communications·G MatthijsP Marynen

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