A cluster of four homologous small RNAs modulates C1 metabolism and the pyruvate dehydrogenase complex in Rhodobacter sphaeroides under various stress conditions

Journal of Bacteriology
Fabian BillenkampGabriele Klug

Abstract

In bacteria, regulatory RNAs play an important role in the regulation and balancing of many cellular processes and stress responses. Among these regulatory RNAs, trans-encoded small RNAs (sRNAs) are of particular interest since one sRNA can lead to the regulation of multiple target mRNAs. In the purple bacterium Rhodobacter sphaeroides, several sRNAs are induced by oxidative stress. In this study, we focused on the functional characterization of four homologous sRNAs that are cotranscribed with the gene for the conserved hypothetical protein RSP_6037, a genetic arrangement described for only a few sRNAs until now. Each of the four sRNAs is characterized by two stem-loops that carry CCUCCUCCC motifs in their loops. They are induced under oxidative stress, as well as by various other stress conditions, and were therefore renamed here sRNAs CcsR1 to CcsR4 (CcsR1-4) for conserved CCUCCUCCC motif stress-induced RNAs 1 to 4. Increased CcsR1-4 expression decreases the expression of genes involved in C1 metabolism or encoding components of the pyruvate dehydrogenase complex either directly by binding to their target mRNAs or indirectly. One of the CcsR1-4 target mRNAs encodes the transcriptional regulator FlhR, an activator of glutathi...Continue Reading

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Citations

Oct 4, 2019·Environmental Microbiology·Matthew McIntoshGabriele Klug
Jan 15, 2020·Journal of Bacteriology·José Hernández-ValleLaura Camarena
Oct 16, 2016·The FEBS Journal·Andrea von ZadowGabriele Klug
May 28, 2021·Frontiers in Molecular Biosciences·Michel Fasnacht, Norbert Polacek
Jun 15, 2021·Nucleic Acids Research·Julian GrütznerGabriele Klug
Jul 25, 2021·International Journal of Molecular Sciences·Katrin M H EisenhardtGabriele Klug

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