A co-culture system of rat synovial stem cells and meniscus cells promotes cell proliferation and differentiation as compared to mono-culture

Scientific Reports
Xing XieYingfang Ao

Abstract

A meniscus tear often happens during active sports. It needs to be repaired or replaced surgically to avoid further damage to the articular cartilage. To address the shortage of autologous meniscal cells, we designed a co-culture system of synovial stem cells (SMSCs) and meniscal cells (MCs) to produce a large cell number and to maintain characteristics of MCs. Different ratios of SMSCs and MCs at 3:1, 1:1, and 1:3 were tested. Mono-culture of SMSCs or MCs served as control groups. Proliferation and differentiation abilities were compared. The expression of extracellular matrix (ECM) genes in MCs was assessed using an ECM array to reveal the mechanism at the gene level. The co-culture system of SMSCs/MCs at the ratio of 1:3 showed better results than the control groups or those at other ratios. This co-culture system may be a promising strategy for meniscus repair with tissue engineering.

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Citations

Mar 30, 2021·Frontiers in Bioengineering and Biotechnology·Maria Rita CiteroniBarbara Barboni

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Methods Mentioned

BETA
flow cytometry
chip
PCR
fluorescence activated cell sorting

Software Mentioned

ModFit LT
Pro Plus ( IPP
BD FACSuite
Image

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