PMID: 9531537May 9, 1998Paper

A coactivator of pre-mRNA splicing

Genes & Development
B J BlencoweP A Sharp

Abstract

The nuclear matrix antigen recognized by the monoclonal antibody (mAb) B1C8 is a novel serine (S) and arginine (R)-rich protein associated with splicing complexes and is named here SRm160 (SR-related matrix protein of 160 kD). SRm160 contains multiple SR repeats, but unlike proteins of the SR family of splicing factors, lacks an RNA recognition motif. SRm160 and a related protein SRm300 (the 300-kD nuclear matrix antigen recognized by mAb B4A11) form a complex that is required for the splicing of specific pre-mRNAs. The SRm160/300 complex associates with splicing complexes and promotes splicing through interactions with SR family proteins. Binding of SRm160/300 to pre-mRNA is normally also dependent on U1 snRNP and is stabilized by U2 snRNP. Thus, SRm160/300 forms multiple interactions with components bound directly to important sites within pre-mRNA. The results suggest that a complex of the nuclear matrix proteins SRm160 and SRm300 functions as a coactivator of pre-mRNA splicing.

References

Apr 10, 1992·Science·M Tian, T Maniatis
May 1, 1992·Genes & Development·A M ZahlerM B Roth
Jul 10, 1992·Cell·U T Meier, G Blobel
Jun 1, 1989·Experimental Cell Research·H C SmithS M Berget
Mar 1, 1989·The Journal of Cell Biology·S ZeitlinA Efstratiadis
Apr 14, 1982·Biochemical and Biophysical Research Communications·B Vogelstein, B F Hunt
Jan 1, 1983·Biology of the Cell·D L SpectorH Busch
Jun 17, 1994·Cell·P A Sharp
Aug 1, 1994·The Journal of Cell Biology·S HuangD L Spector
Jan 1, 1993·Cold Spring Harbor Symposia on Quantitative Biology·J B LawrenceX Xing
Jan 1, 1995·Methods in Molecular Biology·B J Blencowe, S M Barabino
Nov 1, 1994·The Journal of Cell Biology·B J BlencoweP A Sharp
Jan 1, 1993·Annual Review of Cell Biology·D L Spector
Jan 18, 1994·Proceedings of the National Academy of Sciences of the United States of America·K M WanS Penman
Dec 5, 1995·Proceedings of the National Academy of Sciences of the United States of America·M L HedleyT Maniatis
May 1, 1996·The Journal of Cell Biology·S Huang, D L Spector
Jul 1, 1996·Genes & Development·J L Manley, R Tacke
Aug 6, 1996·Proceedings of the National Academy of Sciences of the United States of America·M J MortillaroR Berezney
Apr 1, 1996·Current Opinion in Genetics & Development·R Reed
Aug 1, 1996·Trends in Biochemical Sciences·J Valcárcel, M R Green
Jan 1, 1996·Annual Review of Biochemistry·A Krämer
Nov 1, 1996·Trends in Genetics : TIG·B Chabot
Apr 29, 1997·Proceedings of the National Academy of Sciences of the United States of America·J A NickersonS Penman
May 29, 1997·Nature·T MisteliD L Spector
Jun 6, 1998·Journal of Molecular Biology·T Pederson

❮ Previous
Next ❯

Citations

Sep 27, 2000·Biochimica Et Biophysica Acta·Y SawadaS Ohtaki
Feb 27, 2001·Molecular and Cellular Endocrinology·J HanH Jarry
Apr 26, 2003·Trends in Biochemical Sciences·Hervé Le HirMelissa J Moore
May 14, 1999·Trends in Biochemical Sciences·B J Blencowe, C A Ouzounis
Feb 11, 2000·RNA·B J BlencoweP A Sharp
Sep 6, 2011·Nature Structural & Molecular Biology·Sandra Bento MartinsMaria Carmo-Fonseca
Oct 21, 2003·Molecular Biology of the Cell·Ian CushmanMary Shannon Moore
Dec 18, 2001·EMBO Reports·H Le HirE Izaurralde
Jan 11, 2000·Nucleic Acids Research·W van Der Houven Van OordtJ F Cáceres
Sep 20, 2006·Nucleic Acids Research·A Helena MangsBrian J Morris
Nov 3, 2010·Molecular and Cellular Biology·Christine M Misquitta-AliBenjamin J Blencowe
Dec 29, 2011·Molecular and Cellular Biology·Laetitia MichelleBenoit Chabot
Dec 12, 2001·Molecular and Cellular Biology·Susan McCrackenBenjamin J Blencowe
Apr 1, 2003·Molecular and Cellular Biology·Sònia GuilMontse Bach-Elias
Oct 16, 2004·Molecular and Cellular Biology·Matthew J SmithTony Pawson
Jan 20, 2004·Molecular and Cellular Biology·Eiji SakashitaAkila Mayeda
Mar 31, 2005·Molecular and Cellular Biology·Demian CazallaJavier F Cáceres
Dec 16, 2005·Molecular and Cellular Biology·Chonghui Cheng, Phillip A Sharp
Mar 11, 2003·Annual Review of Biochemistry·Douglas L Black
Jan 10, 2002·The Journal of Clinical Investigation·Peter H Byers
Jan 20, 2004·RNA·Chia C ChanGideon Dreyfuss

❮ Previous
Next ❯

Related Concepts

Related Feeds

Alternative splicing

Alternative splicing a regulated gene expression process that allows a single genetic sequence to code for multiple proteins. Here is that latest research.