A collagen-based corneal stroma substitute with micro-designed architecture

Biomaterials Science
Cemile KilicV Hasirci

Abstract

A 3D corneal stroma substitute with micro-level patterns was constructed from a stack of 4 micro patterned collagen or collagen-elastin like recombinamer (Col:ELR) blend layers. The transparency of all of the films was quite high with the uncrosslinked (UXL) films and dehydrothermally treated (150 °C, 24 h) Col:ELR films yielding the best results. Human corneal keratocytes (HK) could be attached and proliferated equally well on the single films of Col and Col:ELR. However, for the multilayer constructs the proliferation was higher on Col than on Col:ELR. The cells were found to align along the patterns (microchannels with a 39 μm groove depth, 8 μm groove width, 3.3 μm ridge width, and 54.7° inclination angle) of the films, while no significant alignment was observed on the unpatterned films. The transparency of the seeded Col:ELR films was superior to the Col films over a 30 day incubation period and was quite close to that of a native human cornea. It was concluded that the Col and Col:ELR films and their 3D constructs have significant potential for use as corneal stroma substitutes.

References

Mar 1, 1996·Microscopy Research and Technique·R W Beuerman, L Pedroza
Jun 5, 1998·Biomaterials·A Curtis, C Wilkinson
Jul 23, 1999·International Journal of Biological Macromolecules·S F WilliamsO P Peoples
Sep 28, 2000·Micron : the International Research and Review Journal for Microscopy·K M Meek, N J Fullwood
Apr 30, 1957·The Journal of Physiology·D M MAURICE
Apr 27, 2004·Experimental Eye Research·Keith M Meek, Craig Boote
Jul 17, 2004·Annual Review of Biomedical Engineering·C Ross EthierJeff Ruberti
Jun 18, 2005·Brain Research. Brain Research Reviews·Ning ZhangXuejun Wen
Jun 8, 2006·Journal of Biomedical Materials Research. Part a·P ZorlutunaV Hasirci
Dec 13, 2006·Biochemical Pharmacology·Gene L BidwellDrazen Raucher
Jul 10, 2007·Journal of Biomedical Materials Research. Part a·E VranaV Hasirci
Nov 13, 2007·Seminars in Cell & Developmental Biology·James V Jester
Mar 13, 2008·Tissue Engineering. Part a·Rachael A B Crabb, Allison Hubel
Apr 22, 2008·Biotechnology Progress·David MyungCurtis W Frank
Apr 23, 2008·Pediatric Research·Ajay ShahElizabeth Orwin
Aug 19, 2008·Investigative Ophthalmology & Visual Science·N Engin VranaVasif Hasirci
Dec 9, 2008·Biomaterials·Brian D LawrenceDavid L Kaplan
Jan 28, 2009·Current Eye Research·Hernán Martínez-OsorioMargarita Calonge
Apr 21, 2011·Journal of Materials Science. Materials in Medicine·Beste KinikogluVasif Hasirci
May 4, 2011·Tissue Engineering. Part a·Ryou WatanabeKohji Nishida
Sep 5, 2012·Indian Journal of Ophthalmology·Matthew S OlivaManoj Gulati
Nov 1, 2012·Biochemical and Biophysical Research Communications·Jong Hyuk YoonTaehoon G Lee
Nov 7, 2012·Dental Materials : Official Publication of the Academy of Dental Materials·Shruti S Amruthwar, Amol V Janorkar

❮ Previous
Next ❯

Citations

Jul 15, 2015·Advanced Healthcare Materials·Alessandra GirottiJosé Carlos Rodríguez-Cabello
Sep 30, 2015·Journal of Biomaterials Science. Polymer Edition·Esen SayinVasif Hasirci
Nov 6, 2014·Regenerative Medicine·Esen SayinVasif Hasirci
Jul 30, 2015·Biomaterials Science·H M RostamA M Ghaemmaghami
May 29, 2014·Journal of Biomaterials Science. Polymer Edition·A Acun, V Hasirci
Jul 30, 2014·The Journals of Gerontology. Series A, Biological Sciences and Medical Sciences·Ben SchöttkerHermann Brenner
Dec 2, 2017·Journal of Tissue Engineering and Regenerative Medicine·Cemile Kilic Bektas, Vasif Hasirci
Jul 13, 2018·Journal of Biomaterials Science. Polymer Edition·Cemile Kilic BektasNesrin Hasirci
Nov 21, 2019·Biomaterials Science·Cemile Kilic Bektas, Vasif Hasirci
Jan 1, 2015·Beilstein Journal of Organic Chemistry·Anxo Fernández-FerreiroFranciscoJ Otero-Espinar
Dec 8, 2019·Journal of Materials Science. Materials in Medicine·Cemile Kilic Bektas, Vasif Hasirci
May 29, 2020·BioMed Research International·Sören SeganChristopher Shipp
Sep 12, 2019·Journal of Biomaterials Science. Polymer Edition·Cemile Kilic BektasVasif Hasirci
Aug 8, 2020·International Journal of Biological Macromolecules·Alessandra GirottiF Javier Arias
May 12, 2021·International Journal of Biological Macromolecules·Asal Farasatkia, Mahshid Kharaziha
Apr 15, 2021·American Journal of Physiology. Cell Physiology·Adeel AhmedVinay V Abhyankar

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.