A collagen Vα1-derived fragment inhibits FGF-2 induced-angiogenesis by modulating endothelial cells plasticity through its heparin-binding site.

Matrix Biology : Journal of the International Society for Matrix Biology
Tao JiaFlorence Ruggiero

Abstract

Type V collagen (ColV) is a component of the endothelial basement membrane zone. During angiogenesis, extracellular matrix remodelling results in the release of active protein fragments that display pro- or anti-angiogenic properties. The latter often exert their activity through their heparin-binding site. We previously characterized a ColVα1-derived fragment called HEPV that contains a high affinity-binding site for heparin and heparan sulphate chains. Here we show that HEPV binds to FGF2 through its heparin-binding site. Using in vitro and in vivo angiogenesis assays, we show that HEPV but not the HEPV mutant at the heparin-binding site, inhibits FGF2-dependant angiogenesis. On the opposite, HEPV does not bind to VEGFA and has no effect on VEGFA-mediated angiogenesis. In 3D collagen gels, the addition of HEPV abrogates endothelial cell invasion and sprouting induced by FGF2. Interestingly, in vivo experiments reveal that HEPV anti-angiogenic activity is associated with the appearance of endothelial to mesenchymal transition (EndMT) markers. Together, these findings indicate that the ColVα1-derived fragment HEPV functions as an anti-angiogenic factor that represses FGF2-mediated angiogenesis through the regulation of endothel...Continue Reading

References

Apr 15, 2000·The Journal of Biological Chemistry·Y MaeshimaR Kalluri
Jun 23, 2000·The Journal of Biological Chemistry·F DelacouxF Ruggiero
Mar 19, 2003·The Journal of Biological Chemistry·Janelle L Lauer-FieldsGregg B Fields
Apr 20, 2004·Journal of Cellular Biochemistry·Richard J WenstrupDavid E Birk
Jan 18, 2005·Trends in Cell Biology·Gregory Bix, Renato V Iozzo
Jul 4, 2006·The Journal of Biological Chemistry·Sylvie Ricard-BlumFlorence Ruggiero
Jul 25, 2008·Nature Reviews. Cancer·Gabriele Bergers, Douglas Hanahan
Jul 17, 2010·American Journal of Medical Genetics. Part a·Guntram BorckChristian Kubisch
Mar 1, 2010·Biochemistry and Molecular Biology Education : a Bimonthly Publication of the International Union of Biochemistry and Molecular Biology·João Carlos SousaJoana Almeida Palha
May 20, 2011·Nature·Peter Carmeliet, Rakesh K Jain
Oct 12, 2011·Cell and Tissue Research·Vivien J Coulson-ThomasHelena B Nader
Oct 14, 2011·The Journal of Clinical Endocrinology and Metabolism·Michael SpencerPhilip A Kern
Oct 12, 2012·Molecular Imaging and Biology : MIB : the Official Publication of the Academy of Molecular Imaging·Michelle KeramidasJean-Luc Coll
Oct 5, 2013·Molecular Cancer Research : MCR·Rekha GyanchandaniSeungwon Kim
Nov 20, 2013·Matrix Biology : Journal of the International Society for Matrix Biology·Franck Peysselon, Sylvie Ricard-Blum
Feb 21, 2014·Journal of Cell Science·Katrina M Welch-ReardonChristopher C W Hughes
Feb 24, 2015·Matrix Biology : Journal of the International Society for Matrix Biology·Renato V Iozzo, Liliana Schaefer
Apr 8, 2015·American Journal of Medical Genetics. Part a·Glen R MonroeAnnette F Baas
Feb 13, 2016·Frontiers in Pharmacology·Sylvie Ricard-Blum, Sylvain D Vallet
Nov 4, 2016·International Journal of Molecular Sciences·Maurizio MongiatAlice Paulitti
Jan 4, 2017·Matrix Biology : Journal of the International Society for Matrix Biology·Ambra PozziRenato V Iozzo
Jan 15, 2017·Cancer Treatment Reviews·Kim C AaldersFatima Cardoso
Jan 20, 2017·Nature Communications·Guorui HuangDaniel S Greenspan
Feb 10, 2017·Nature Communications·Elisabetta DejanaMichael Simons
Nov 13, 2017·Matrix Biology : Journal of the International Society for Matrix Biology·Achilleas D Theocharis, Nikos K Karamanos
Mar 4, 2018·Drug Resistance Updates : Reviews and Commentaries in Antimicrobial and Anticancer Chemotherapy·Rajesh N Gacche, Yehuda G Assaraf
Apr 7, 2018·Matrix Biology : Journal of the International Society for Matrix Biology·Renato V Iozzo, Maria A Gubbiotti
May 2, 2018·Matrix Biology : Journal of the International Society for Matrix Biology·Fransiska Malfait
Aug 22, 2018·Matrix Biology : Journal of the International Society for Matrix Biology·Nikos K KaramanosRenato V Iozzo
Feb 26, 2019·Matrix Biology : Journal of the International Society for Matrix Biology·Stavros Garantziotis, Rashmin C Savani
Jun 21, 2019·Matrix Biology : Journal of the International Society for Matrix Biology·Jarkko KoivunenTaina Pihlajaniemi

❮ Previous
Next ❯

Citations

Sep 21, 2020·Matrix Biology : Journal of the International Society for Matrix Biology·Mei SunDavid E Birk
Feb 27, 2021·Journal of Cellular Physiology·Ali DakroubAli H Eid
Jul 28, 2021·Matrix Biology : Journal of the International Society for Matrix Biology·Prashant ChandrasekaranLin Han

❮ Previous
Next ❯

Related Concepts

Related Feeds

Arterial-Venous in Development & Disease

Arterial-venous development may play a crucial role in cardiovascular diseases. Here is the latest research.

Related Papers

The Journal of Biological Chemistry
Sylvie Ricard-BlumFlorence Ruggiero
The Journal of Biological Chemistry
F DelacouxFlorence Ruggiero
The International Journal of Biochemistry & Cell Biology
R I VijiP R Sudhakaran
© 2021 Meta ULC. All rights reserved