A collagenous protective coat enables Metarhizium anisopliae to evade insect immune responses.

Proceedings of the National Academy of Sciences of the United States of America
Chengshu Wang, Raymond J St Leger

Abstract

The ubiquitous fungal pathogen Metarhizium anisopliae kills a wide range of insects. Host hemocytes can recognize and ingest its conidia, but this capacity is lost on production of hyphal bodies. We show that the unusual ability of hyphal bodies to avoid detection depends on a gene (Mcl1) that is expressed within 20 min of the pathogen contacting hemolymph. A mutant disrupted in Mcl1 is rapidly attacked by hemocytes and shows a corresponding reduction of virulence to Manduca sexta. Mcl1 encodes a three domain protein comprising a hydrophilic, negatively charged N-terminal region with 14 cysteine residues, a central region comprising tandem repeats (GXY) characteristic of collagenous domains, and a C-terminal region that includes a glycosylphosphatidylinositol-dependent cell wall attachment site. Immunofluorescence assay showed that hyphal bodies are covered by the N-terminal domains of MCL1. The collagen domain became antibody accessible after treatment with DTT, suggesting that the N termini are linked by interchain disulfide bonds and are presented on the cell surface by extended collagenous fibers. Studies with staining reagents and hemocyte monolayers showed that MCL1 functions as an antiadhesive protective coat because it ...Continue Reading

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Citations

Jun 18, 2009·Die Naturwissenschaften·Hasan Tunaz, David Stanley
Oct 29, 2009·Applied Microbiology and Biotechnology·Raymond J St Leger, Chengshu Wang
Apr 30, 2008·Current Microbiology·Xing-gang LiaoYan Pei
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Dec 7, 2011·Proceedings of the National Academy of Sciences of the United States of America·Sibao WangRaymond J St Leger
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May 9, 2014·Applied Microbiology and Biotechnology·Xinggang LiaoRaymond J St Leger
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