PMID: 8580199Jul 1, 1995

A color image analysis method for assessment of germination based on differential fluorescence staining of bacterial spores and vegetative cells using acridine orange

Biotechnic & Histochemistry : Official Publication of the Biological Stain Commission
J G Bruno, M W Mayo


Color fluorescence image analysis of acridine orange (AO) stained germinating Bacillus subtilis var. niger bacteria revealed a cell population initially dominated by small green spores followed by the emergence of at least three additional discernible subpopulations in response to stimulation with D-glucose. These subpopulations were small, round or oblong red cells; intermediate to large metachromatic cells; and large red rods. Large green rods were rarely observed. An increase in red emissions (i.e., putative RNA synthesis) was sometimes seen as early as 90 min after exposure to D-glucose and uptake of AO at room temperature. This may represent either metabolic recovery from quiescence or RNA synthesis associated with germination. In the absence of D-glucose, or using autoclaved bacteria in the presence of glucose, no relative increase in the red signal was observed despite hours of observation. Digital image analysis was used for relative measurement of red, green and blue signals and to correlate the size of various subpopulations with their fluorescence color emissions over time. Image analysis demonstrated a trend toward increasing size and red emission in the presence of glucose. The average red emission was found to be ...Continue Reading


May 1, 1979·Journal of Bacteriology·F M RacineJ C Vary
Jan 18, 1978·Biochimica Et Biophysica Acta·L K Shay, J C Vary
May 1, 1976·Proceedings of the National Academy of Sciences of the United States of America·N H Mendelson
Jan 1, 1992·Biotechnic & Histochemistry : Official Publication of the Biological Stain Commission·D K Sharma, D N Prasad
Sep 1, 1990·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·J Kapuscinski
Apr 1, 1991·Journal of Clinical Microbiology·M D Miliotis
Jul 1, 1991·The Journal of Applied Bacteriology·J P Back, R G Kroll
Oct 1, 1991·Neurology·A M HegartyK Freeman
Dec 1, 1987·The Journal of Applied Bacteriology·A F Kelly, R G Kroll
Apr 1, 1972·Journal of Bacteriology·C PrasadE Freese
Apr 1, 1972·Journal of Bacteriology·A D HitchinsR A Slepecky
Mar 1, 1966·Journal of Bacteriology·H F Foerster, J W Foster
Jan 1, 1968·Proceedings of the National Academy of Sciences of the United States of America·R L Armstrong, N SUEOKA
Mar 1, 1980·Journal of Bacteriology·T MaruyamaC Umezawa
Jul 15, 1981·The Biochemical Journal·G S StewartD J Ellar


May 1, 1996·Biotechnic & Histochemistry : Official Publication of the Biological Stain Commission·J G BrunoP J Stopa
Feb 19, 2002·Letters in Applied Microbiology·T HamoudaJ R Baker
Jan 18, 2006·Letters in Applied Microbiology·L ZhaoD W Schaffner
Mar 11, 2006·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·Maria Cristina AlbertiniBarbara Citterio

Related Concepts

C.I. Basic Orange 14
Natto Bacteria
Optical Image Reconstruction
Microscopy, Phase-Contrast
Fluorescence Spectroscopy
Spores, Bacterial

Trending Feeds


Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Alzheimer's Disease: MS4A

Variants within the membrane-spanning 4-domains subfamily A (MS4A) gene cluster have recently been implicated in Alzheimer's disease in genome-wide association studies. Here is the latest research on Alzheimer's disease and MS4A.

Pediculosis pubis

Pediculosis pubis is a disease caused by a parasitic insect known as Pthirus pubis, which infests human pubic hair, as well as other areas with hair including eye lashes. Here is the latest research.

Rh Isoimmunization

Rh isoimmunization is a potentially preventable condition that occasionally is associated with significant perinatal morbidity or mortality. Discover the latest research on Rh Isoimmunization here.

Genetic Screens in iPSC-derived Brain Cells

Genetic screening is a critical tool that can be employed to define and understand gene function and interaction. This feed focuses on genetic screens conducted using induced pluripotent stem cell (iPSC)-derived brain cells. It also follows CRISPR-Cas9 approaches to generating genetic mutants as a means of understanding the effect of genetics on phenotype.

Enzyme Evolution

This feed focuses on molecular models of enzyme evolution and new approaches (such as adaptive laboratory evolution) to metabolic engineering of microorganisms. Here is the latest research.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Pharmacology of Proteinopathies

This feed focuses on the pharmacology of proteinopathies - diseases in which proteins abnormally aggregate (i.e. Alzheimer’s, Parkinson’s, etc.). Discover the latest research in this field with this feed.

Alignment-free Sequence Analysis Tools

Alignment-free sequence analyses have been applied to problems ranging from whole-genome phylogeny to the classification of protein families, identification of horizontally transferred genes, and detection of recombined sequences. Here is the latest research.

Related Papers

Biotechnic & Histochemistry : Official Publication of the Biological Stain Commission
J G BrunoP J Stopa
Biotechnic & Histochemistry : Official Publication of the Biological Stain Commission
D K Sharma, D N Prasad
Cytometry. Part a : the Journal of the International Society for Analytical Cytology
Maria C AlbertiniMarco B L Rocchi
© 2021 Meta ULC. All rights reserved