PMID: 8580199Jul 1, 1995

A color image analysis method for assessment of germination based on differential fluorescence staining of bacterial spores and vegetative cells using acridine orange

Biotechnic & Histochemistry : Official Publication of the Biological Stain Commission
J G Bruno, M W Mayo

Abstract

Color fluorescence image analysis of acridine orange (AO) stained germinating Bacillus subtilis var. niger bacteria revealed a cell population initially dominated by small green spores followed by the emergence of at least three additional discernible subpopulations in response to stimulation with D-glucose. These subpopulations were small, round or oblong red cells; intermediate to large metachromatic cells; and large red rods. Large green rods were rarely observed. An increase in red emissions (i.e., putative RNA synthesis) was sometimes seen as early as 90 min after exposure to D-glucose and uptake of AO at room temperature. This may represent either metabolic recovery from quiescence or RNA synthesis associated with germination. In the absence of D-glucose, or using autoclaved bacteria in the presence of glucose, no relative increase in the red signal was observed despite hours of observation. Digital image analysis was used for relative measurement of red, green and blue signals and to correlate the size of various subpopulations with their fluorescence color emissions over time. Image analysis demonstrated a trend toward increasing size and red emission in the presence of glucose. The average red emission was found to be ...Continue Reading

References

May 1, 1979·Journal of Bacteriology·F M RacineJ C Vary
Jan 18, 1978·Biochimica Et Biophysica Acta·L K Shay, J C Vary
May 1, 1976·Proceedings of the National Academy of Sciences of the United States of America·N H Mendelson
Jan 1, 1992·Biotechnic & Histochemistry : Official Publication of the Biological Stain Commission·D K Sharma, D N Prasad
Sep 1, 1990·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·J Kapuscinski
Apr 1, 1991·Journal of Clinical Microbiology·M D Miliotis
Jul 1, 1991·The Journal of Applied Bacteriology·J P Back, R G Kroll
Oct 1, 1991·Neurology·A M HegartyK Freeman
Dec 1, 1987·The Journal of Applied Bacteriology·A F Kelly, R G Kroll
Apr 1, 1972·Journal of Bacteriology·C PrasadE Freese
Apr 1, 1972·Journal of Bacteriology·A D HitchinsR A Slepecky
Mar 1, 1966·Journal of Bacteriology·H F Foerster, J W Foster
Jan 1, 1968·Proceedings of the National Academy of Sciences of the United States of America·R L Armstrong, N SUEOKA
Mar 1, 1980·Journal of Bacteriology·T MaruyamaC Umezawa
Jul 15, 1981·The Biochemical Journal·G S StewartD J Ellar

Citations

May 1, 1996·Biotechnic & Histochemistry : Official Publication of the Biological Stain Commission·J G BrunoP J Stopa
Feb 19, 2002·Letters in Applied Microbiology·T HamoudaJ R Baker
Jan 18, 2006·Letters in Applied Microbiology·L ZhaoD W Schaffner
Mar 11, 2006·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·Maria Cristina AlbertiniBarbara Citterio

Related Concepts

C.I. Basic Orange 14
Natto Bacteria
Optical Image Reconstruction
Microscopy, Phase-Contrast
Fluorescence Spectroscopy
Spores, Bacterial

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