A colorimetric assay for S-adenosylhomocysteine hydrolase

Journal of Biochemical and Biophysical Methods
J D Lozada-RamírezF García-Carmona

Abstract

A colorimetric method for S-adenosyl-L-homocysteine hydrolase (SAHase) which uses S-adenosyl-L-homocysteine (SAH) as substrate is described. This method involves the hydrolytic conversion of SAH into adenosine (ADO) and L-homocysteine (HCY). The formation of HCY is quantified using Ellman's reagent and spectrophotometrical measured at 412 nm. Under these assay conditions, the product was followed continuously in a facile and quantitative manner until substrate conversion was complete. This method is an easy, cheap and shorter alternative to more complex methods and it is applicable to routine clinical analysis and in the assay and development of new S-nucleosidylhomocysteines to be used as therapeutic compounds.

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Citations

Mar 16, 2012·Analytical Chemistry·Emmanuel S BurgosVern L Schramm
Oct 31, 2012·PloS One·Claudia AnetzbergerKirsten Jung
Oct 15, 2013·Eukaryotic Cell·Devaraja G Mudeppa, Pradipsinh K Rathod
Jun 27, 2009·Molecular Microbiology·Claudia AnetzbergerKirsten Jung
Sep 26, 2008·Protein Science : a Publication of the Protein Society·Manchi C M ReddyJames C Sacchettini
Nov 28, 2012·Biochemical and Biophysical Research Communications·Rosa Milagros CorralesLaurent Kremer
Jun 15, 2007·FEMS Microbiology Letters·Vlasta CtrnáctáIvan Hrdý
Sep 22, 2017·Journal of Enzyme Inhibition and Medicinal Chemistry·Weiwei HaoJinsong Gu
Oct 29, 2013·Chemical & Pharmaceutical Bulletin·Xiangduan TanGuoping Wang
Jan 5, 2019·Proceedings of the National Academy of Sciences of the United States of America·Jorge Ripoll-RozadaNuno Empadinhas
Dec 6, 2019·Chembiochem : a European Journal of Chemical Biology·Richard DemmlerDirk Hoffmeister
Jun 28, 2019·Journal of Medicinal Chemistry·Ji-Seong YoonLak Shin Jeong
Sep 26, 2012·Journal of Agricultural and Food Chemistry·Inmaculada Navarro-GonzálezFrancisco García-Carmona
Sep 26, 2008·Bioorganic & Medicinal Chemistry·Yang-Ming ZhangFa-Jun Nan

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