A colorimetric assay for the simultaneous measurement of plasminogen activators and plasminogen activator inhibitors in serum-free conditioned media from cultured cells

Analytical Biochemistry
P LeprinceG Moonen

Abstract

The coupled photometric assay for plasminogen activator reported by Coleman and Green (1981) Methods in Enzymology (Lorand, L., Ed.), Vol. 80, pp. 408-414, Academic Press, San Diego, CA) has been adapted for use with 96-well plates and an automatic microplates spectrophotometer. The assay allows the discrimination between tissue-type and urokinase-type plasminogen activators in cell culture-conditioned media. It provides a level of detection of these enzymes in the range 10(-17) to 10(-13) mol (determined using purified human plasminogen activators), uses no radioisotopes, and is faster and more economical than similar assays using specific peptide substrates for plasminogen activators. Levels of free plasminogen activator inhibitor activity can be simultaneously measured on the same samples by a simple adaptation of the assay. This method allows an easy treatment of the data by interfacing with a computer and should thus be useful when large numbers of samples are assayed.

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Citations

Dec 1, 1992·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·F W FawthropR G Russell
Sep 1, 1996·Talanta·J M Fernández-Romero, M D Luque de Castro
Mar 30, 2006·Journal of Cardiopulmonary Rehabilitation·Adam T deJongBarry A Franklin
Feb 26, 1998·American Journal of Respiratory and Critical Care Medicine·C G LardotD F Lison
Jul 19, 2002·International Journal of Cancer. Journal International Du Cancer·Khalid BajouJean-Michel Foidart
Aug 6, 2005·Thrombosis Research·Christopher J WomackGerald L Davis
Mar 23, 2005·Osteoarthritis and Cartilage·P LavigneJ C Fernandes
Oct 3, 2003·Medicine and Science in Sports and Exercise·Christopher J WomackBarry A Franklin

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