A colorimetric assay for viral agents that produce cytopathic effects

Journal of Virological Methods
Caryn L HeldtRuben G Carbonell

Abstract

Many animal viruses produce cytopathic effects in their host cells during a productive infection. While some virus infections can be assayed by the production of plaques, many viruses, while producing cytotoxicity, do not easily form plaques, or do not form plaques at all. Additionally, viruses within families such as the parvoviruses may have different preferred forms of titration making comparative virology difficult even among related groups. Porcine parvovirus (PPV), canine parvovirus (CPV), and minute virus of mice (MVM) are usually titrated using different infectivity assays. A direct comparison of infectious virus titer between these parvoviruses was sought, and a tetrazolium salt assay, MTT has been applied to measure cytopathic effect produced by viral infection for different members of the parvovirus family. Infectious PPV measured using the MTT and the TCID50 assays exhibited excellent correlation and titers for CPV and MVM were consistently duplicated using the MTT assay. The MTT assay was also applied to an unrelated virus, Sindbis, which is routinely titrated by plaque assay. MTT titration of Sindbis virus mutants was found to be valuable for preliminary screening. This assay can be adapted, by correlation to an a...Continue Reading

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Citations

Dec 3, 2013·Indian Journal of Virology : an Official Organ of Indian Virological Society·Hamid Reza PourianfarLara Grollo
Oct 15, 2011·Biologicals : Journal of the International Association of Biological Standardization·Mirta Weber SušanjSvjetlana Terzić
May 2, 2009·Journal of Virological Methods·Liisa LehtorantaThedi Ziegler
Jul 14, 2011·Biotechnology Journal·Caryn L HeldtRuben G Carbonell
May 20, 2015·Journal of Immunology Research·Nohemí Salinas-JazmínSonia Mayra Pérez-Tapia
Jul 9, 2014·Journal of Biotechnology·Maria F GencogluCaryn L Heldt
May 8, 2013·Antiviral Research·Maria F TafurCaryn L Heldt
Sep 18, 2013·Carbohydrate Research·Bingyu BaiCaryn L Heldt
Feb 25, 2014·Carbohydrate Research·Xue MiCaryn L Heldt
Sep 5, 2013·Analytical Chemistry·Stefano MenegattiRuben Guillermo Carbonell
Dec 21, 2017·Viruses·István MészárosZoltán Zádori
May 3, 2018·Biotechnology Progress·Caryn L HeldtMajid Ghafarian
Feb 5, 2010·Current Protocols in Microbiology·Raquel HernandezDennis T Brown
Apr 1, 2014·Clean : Soil, Air, Water·Atheesha GaneshMoganavelli Singh
Aug 23, 2019·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Pratik U JoshiCaryn L Heldt
Oct 21, 2020·Biomaterials Science·Xue MiSarah L Perry
Jul 3, 2020·Langmuir : the ACS Journal of Surfaces and Colloids·Sanaz HabibiAdrienne R Minerick
Mar 23, 2021·PLoS Neglected Tropical Diseases·Nicholas A BergrenRebekah C Kading
Apr 21, 2021·Biotechnology Journal·Christa L MeingastCaryn L Heldt
Jun 16, 2021·Biotechnology and Bioengineering·Pratik U JoshiCaryn L Heldt
Aug 3, 2021·Chemistry of Materials : a Publication of the American Chemical Society·Pegah Kord ForooshaniBruce P Lee
Dec 18, 2019·Langmuir : the ACS Journal of Surfaces and Colloids·Xue MiCaryn L Heldt

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