A colorimetric assay to evaluate the cytotoxic activity of the intestinal intraepithelial lymphocytes of chickens

Veterinary Research Communications
P A Kumar, S K Das

Abstract

After the successful use of 3-[4,5-(dimethylthiazol-2-yl)]-2,5-diphenyltetrazolium bromide (MTT) in cell proliferation assays, its use has been established by different workers in cytotoxicity assays and research on leukaemia. In the present study, a colorimetric assay using MTT was adopted to evaluate the cytotoxic activity of chicken intestinal intraepithelial lymphocytes (iIELs), which constitute an important cellular component of the gut-associated lymphoid tissue (GALT). These iIELs are found to exhibit natural killer (NK) cell-like cytotoxic activity, which is spontaneous, non-MHC-restricted, and does not need to be primed. Hitherto, conventional chromium-release assays have been used to evaluate the cytotoxic activity of iIELs, but these assays have disadvantages such as radiation hazards and loss of the cells in washing steps. The mean percentage cytotoxic activity of chicken iIELs evaluated by the colorimetric assay was 90.37 +/- 2.53 in a group of 5-week-old chickens and 80.2 +/- 3.45 in a group of 8-week-old chickens. These findings established the successful use of a colorimetric assay using MTT for evaluating the cytotoxic activity of chickens iIELs.

References

Mar 1, 1992·Veterinary Immunology and Immunopathology·H S Lillehoj, K S Chung
Oct 1, 1990·Veterinary Immunology and Immunopathology·T J Myers, K A Schat
Sep 1, 1993·Avian Pathology : Journal of the W.V.P.A·L HoggenmuellerK A Schat

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Citations

Jul 19, 2002·Gastroenterology Clinics of North America·Scott Plevy

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