A colorimetric biosensor for simultaneous ochratoxin A and aflatoxins B1 detection in agricultural products

Food Chemistry
Weiran ZhuKun Wang

Abstract

Colorimetric biosensors have been widely applied to mycotoxins testing. However, the colorimetric assay previously reported used a single color to detect one mycotoxin, and there were few reports on the simultaneous detection of multiple mycotoxins. In this work, a colorimetric biosensor for dual mycotoxins detection was developed. A Fe3O4/GO based platform for aflatoxins B1 (AFB1) detection and a Fe3O4@Au based platform for ochratoxin A (OTA) detection were fabricated. The quantification of OTA and AFB1 was respectively achieved by the release of thymolphthalein under alkaline conditions and 3,3',5,5'-tetramethylbenzidine was catalyzed by Au NPs under acidic conditions. Because of different conditions, two sensing methods didn't interfere with each other but could provide a higher detection efficiency. The detection range of AFB1 is 5-250 ng·ml-1 and that of OTA is 0.5-80 ng·ml-1. This biosensor has been successfully applied in real sample detection, which has a broad application prospect in fields of food safety.

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Citations

Sep 16, 2020·Analytical Methods : Advancing Methods and Applications·Marta Jarczewska, Elżbieta Malinowska
Jun 25, 2021·Advanced Healthcare Materials·Kaiyi ZhangXiaodong Chen
Sep 28, 2021·Critical Reviews in Food Science and Nutrition·Md Mehedi HassanQuansheng Chen
Oct 12, 2021·Journal of Animal Science and Biotechnology·Runxian LiPingli He
Nov 4, 2021·Comprehensive Reviews in Food Science and Food Safety·Yujiao HouWeijun Kong

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