A colorimetric lead biosensor using DNAzyme-directed assembly of gold nanoparticles

Journal of the American Chemical Society
Juewen Liu, Yi Lu

Abstract

A highly sensitive and selective colorimetric lead biosensor based on DNAzyme-directed assembly of gold nanoparticles is reported. It consists of a DNAzyme and its substrate that can hybridize to a 5'-thio-modified DNA attached to gold nanoparticles. The hybridization brings gold nanoparticles together, resulting in a blue-colored nanoparticle assembly. In the presence of lead, the DNAzyme catalyzes specific hydrolytic cleavage, which prevents the formation of the nanoparticle assembly, resulting in red-colored individual nanoparticles. The detection level can be tuned to several orders of magnitude, from 100 nM to over 200 muM, through addition of an inactive variant of the DNAzyme. The concept developed here can be applied to the design of nucleic acid enzyme/nanoparticle sensors for analytes that are subject to in vitro selection, and thus can significantly expand the scope of nanomaterial applications and provide a novel approach to designing simple colorimetric biosensors.

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Citations

Dec 29, 2006·Chemistry & Biodiversity·Frank SeelaDingyong Zhong
Dec 1, 2007·Analytical and Bioanalytical Chemistry·Manjula Rajendran, Andrew D Ellington
Dec 18, 2008·Analytical and Bioanalytical Chemistry·Weiming Zheng, Lin He
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Jun 2, 2012·ACS Applied Materials & Interfaces·Mohammed I ShukoorWeihong Tan
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Mar 24, 2009·Chemical Reviews·Juewen LiuYi Lu
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Dec 24, 2013·Inorganic Chemistry·Yu Xiang, Yi Lu
Apr 13, 2006·Journal of the American Chemical Society·Min Su HanChad A Mirkin
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