A colourimetric sensor for the simultaneous determination of oxidative status and antioxidant activity on the same membrane: N,N-dimethyl-p-phenylene diamine (DMPD) on Nafion

Analytica Chimica Acta
Sema Demirci ÇekiçReşat Apak

Abstract

A colourimetric sensor capable of simultaneously measuring oxidative status (OS) in terms of the hazard produced by reactive oxygen species (ROS) and antioxidant activity (AOA) in regard to ROS-scavenging ability of antioxidant compounds was developed. The coloured cationic semi-quinone derivatives, caused by ROS oxidative degradation of N,N-dimethyl-p-phenylene diamine hydrochloride (DMPD) in pH 5.7 acetate-buffered medium, were formed in solution and immobilized on a perfluorosulfonate-based Nafion membrane. ROS, namely hydroxyl (·OH) and superoxide (O2(·-)) radicals, were produced by Fenton/UV and xanthine/xanthine oxidase methods, respectively. The pink-coloured, (+)-charged chromophore (referred to as DMPD-quinone or DMPDQ), resulting from the reaction between DMPD and ROS, could be completely retained on the solid membrane sensor by electrostatic interaction with the anionic sulfonate groups of Nafion. After equilibration, the Nafion membrane surface was homogeneously coloured enabling an absorbance measurement at 514 nm, while the aqueous phase completely lacked colour. Antioxidants, when present, caused an absorbance decrease on the membrane due to their ROS scavenging action, giving rise to less DMPDQ production. The a...Continue Reading

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May 8, 2013·Journal of Agricultural and Food Chemistry·Sema Demirci ÇekiçReşat Apak

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Citations

Jul 17, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Zdenka BedlovičováAneta Salayová
Nov 11, 2020·Analytical Methods : Advancing Methods and Applications·Reşat ApakSelen Durmazel
Jul 2, 2019·Journal of Agricultural and Food Chemistry·Reşat Apak

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