A combination of astragaloside I, levistilide A and calycosin exerts anti-liver fibrosis effects in vitro and in vivo

Acta Pharmacologica Sinica
Tao GuoChenghai Liu

Abstract

Liver fibrosis is excessive accumulation of extracellular matrix proteins that results from various chronic liver diseases. Hepatic stellate cells (HSCs) play an essential role in the pathogenesis of liver fibrosis. Danggui Buxue Tang (DBT) is a classic formula of Chinese traditional medicine. We previously showed that DBT could ameliorate liver fibrosis in rats. However, the bioactive components of DBT in the treatment of liver fibrosis remain unknown. In this study we evaluated 14 ingredients from DBT in human hepatic stellate cell line LX-2, and found that astragaloside I (A), levistilide A (L) and calycosin (C) produced synergistic proliferation inhibition on LX-2 cells and TGF-β1-activated LX-2 cells. Thus, we prepared a mixture of them, and named this combination as ALC formula. Using high-content screening and Western blot assay we revealed that the ALC formula significantly reduced the expression of α-SMA and collagen I in LX-2 cells. The in vivo anti-fibrosis effects of ALC formula were evaluated in a liver fibrosis model in C57BL/6 mice established through injection of dimethylnitrosamine (DMN 2 mg/kg, ip) for 4 weeks. In the third week, the nice were injected with ALC formula (astragaloside I 44.21 mg/kg per day, lev...Continue Reading

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Citations

Apr 12, 2019·Combinatorial Chemistry & High Throughput Screening·Xiang LiuYing Zhang
Jul 2, 2020·Drug Design, Development and Therapy·Danyan LiShengsheng Zhang
Dec 22, 2020·Expert Review of Anti-infective Therapy·Mao DengXiaofang Li
Jan 26, 2021·Oxidative Medicine and Cellular Longevity·Yu FuYonggui He
Sep 29, 2021·Chinese Journal of Integrative Medicine·Xin SunCheng-Hai Liu

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Methods Mentioned

BETA
protein assay
electrophoresis
Fluorescence

Software Mentioned

Cellomics Cell Health Profiling Bio Application
SmartView
SPSS

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