A combination of fluorescent probes for evaluation of cytotoxicity and toxic mechanisms in isolated rainbow trout hepatocytes

Toxicology in Vitro : an International Journal Published in Association with BIBRA
H LiliusB Isomaa

Abstract

The toxicity of 17 chemicals to freshly isolated rainbow trout hepatocytes was determined using four different fluorescent probes measuring different endpoints. Calcein was used to determine viability of the cells, rhodamine 123 and 5,5',6,6'-tetrachloro-1,1',3,3'-tetraethylbenzimidazolylcarbocyanine iodide (JC-1) to monitor mitochondrial activity and 5-chloromethylfluorescein diacetate (CMFDA) to monitor intracellular glutathione (GSH) levels. An EC(50) value 10 times lower than that obtained with the calcein assay was taken to indicate a specific action. Two sets of test chemicals were used. The first set contained five chemicals with a known specific toxic action and two chemicals without a known main specific action at a cellular level, and this set was used to examine the specificity of the probes. The second set consisted of 10 chemicals from the MEIC (Multicenter Evaluation of In Vitro Cytotoxicity) chemical list and included only one chemical with a known main specific action at a cellular level. In the first set of chemicals the CMFDA assay detected all chemicals with known GSH-depleting action, and the results obtained with the second set of chemicals indicated that none of the chemicals act mainly by GSH depletion. H...Continue Reading

References

Feb 1, 1992·Toxicology and Applied Pharmacology·R C BurghardtT D Phillips
Jan 1, 1991·Journal of Biochemical Toxicology·M Z Badr
Mar 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·A JainA Meister
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Jun 1, 1995·Toxicology in Vitro : an International Journal Published in Association with BIBRA·H LiliusB Isomaa

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Citations

Aug 1, 1999·Toxicology in Vitro : an International Journal Published in Association with BIBRA·S RappeneauF Marano
Oct 16, 2009·Brain Research Bulletin·Jonas PetterssonLiudmila N Novikova
May 16, 2009·Toxicology in Vitro : an International Journal Published in Association with BIBRA·A JosH Segner
Nov 29, 2005·Comparative Biochemistry and Physiology. Toxicology & Pharmacology : CBP·Xiao-yong ChenXin-mei Liu
Jul 17, 2018·Environmental Sciences Europe·Kristina RehbergerHelmut Segner
Feb 6, 2021·Scientific Reports·Kristina RehbergerHelmut Segner

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