A Combination Therapy of 17β-Estradiol and Memantine Is More Neuroprotective Than Monotherapies in an Organotypic Brain Slice Culture Model of Traumatic Brain Injury

Journal of Neurotrauma
Michael R Lamprecht, Barclay Morrison

Abstract

Combination therapies are a promising therapeutic option for traumatic brain injury (TBI) owing to the clinical failure of monotherapy treatments, such as progesterone. Organotypic hippocampal slice cultures (OHSCs) from Sprague-Dawley rats were subjected to an in vitro TBI, and the neuroprotective effects of 17β-estradiol (E2) or memantine (MEM) monotherapies were quantified. Several combination treatments at different concentrations of both drugs were tested, with 100 pM of E2 and 10 μM of MEM statistically and significantly reducing cell death over either monotherapy when administered immediately after injury. This combination was also significantly neuroprotective when administered 1 h postinjury, possibly supporting future in vivo studies. Further, we hypothesized that this synergy could be the result of MEM blocking a potentially deleterious effect of E2, specifically E2 enhancement of N-methyl-D-aspartate (NMDA) currents. Evoked electrophysiological responses in OHSCs were potentiated by E2 treatment, whereas this potentiation was significantly reduced by MEM. In conclusion, a combination therapy of E2 and memantine was significantly more neuroprotective than both monotherapy treatments, and this synergy may be the resul...Continue Reading

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Citations

Sep 6, 2017·Canadian Journal of Physiology and Pharmacology·Soudabeh NaderiMohammad Allahtavakoli
Jun 10, 2019·Restorative Neurology and Neuroscience·Eric E AbrahamsonMilos D Ikonomovic
Jul 3, 2021·Advanced Functional Materials·Michael L LovettDavid L Kaplan

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Methods Mentioned

BETA
hormone replacement therapies

Software Mentioned

MATLAB
MotionPro
MATLAB scripts
RESCUE
MATLAB script
Metamorph

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Brain Injury & Trauma

brain injury after impact to the head is due to both immediate mechanical effects and delayed responses of neural tissues.