A combined adsorption-gel filtration technique for the determination of the cortisol-binding capacity of transcortin.

Zeitschrift Für Klinische Chemie Und Klinische Biochemie
U Schwartz, J Hammerstein

Abstract

A combined adsorption-gel filtration technique has been developed for the quantitation of the cortisol-binding capacity of transcortin: Endogenous steroids are removed from plasma by adsorption on uncoated charcoal. Saturation of the "stripped" binding sites of transcortin is accomplished by equilibrating the sample with a definite amount of labeled cortisol of low specific activity (0.1 muCi/mug). Transcortin-bound [4-14C]cortisol is isolated by gel filtration over Sephadex G-50 at 4 degrees C and measured by liquid scintillation counting. The cortisol-binding capacity of transcortin is calculated directly on the basis of the known specific activity of cortisol. The modification described eliminates methodological disadvantages associated with the original gel filtration procedures, i.e. the possible interference of various endogenous steroids with cortisol binding to transcortin, and the necessity of fluorometric or colorimetric determination of protein-bound cortisol. The values of the cortisol-binding capacity of transcortin in plasma obteined by this simplified assay are in close agreement with results reported in the literature (mean +/- S.D.): healthy males 261 +/- 23 mug/l) of transcortin-bound cortisol (n = 13), health...Continue Reading

References

Sep 1, 1965·The Journal of Clinical Endocrinology and Metabolism·B U MusaR P Doe
Jan 1, 1970·Acta Endocrinologica. Supplementum·W R Slaunwhite, A A Sandberg
Apr 1, 1962·The Journal of Clinical Investigation·P DE MOORM DECLERCK-RASKIN
May 1, 1963·The Journal of Clinical Endocrinology and Metabolism·B P MURPHY, C J PATTEE
Sep 1, 1963·Acta Endocrinologica·P DEMOORR DECKX
Oct 1, 1964·The Journal of Clinical Endocrinology and Metabolism·R P DOEU S SEAL
Oct 1, 1964·The Journal of Clinical Endocrinology and Metabolism·C OSORIO, D L SCHATS
May 1, 1962·British Medical Bulletin·I H MILLS

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