A combined cell-consortium approach for lignocellulose degradation by specialized Lactobacillus plantarum cells

Biotechnology for Biofuels
Sarah MoraïsItzhak Mizrahi

Abstract

Lactobacillus plantarum is an attractive candidate for metabolic engineering towards bioprocessing of lignocellulosic biomass to ethanol or polylactic acid, as its natural characteristics include high ethanol and acid tolerance and the ability to metabolize the two major polysaccharide constituents of lignocellulolytic biomass (pentoses and hexoses). We recently engineered L. plantarum via separate introduction of a potent cellulase and xylanase, thereby creating two different L. plantarum strains. We used these strains as a combined cell-consortium for synergistic degradation of cellulosic biomass. To optimize enzymatic degradation, we applied the cell-consortium approach to assess the significance of enzyme localization by comparing three enzymatic paradigms prevalent in nature: (i) a secreted enzymes system, (ii) enzymes anchored to the bacterial cell surface and (iii) enzymes integrated into cellulosome complexes. The construction of the three paradigmatic systems involved the division of the production and organization of the enzymes and scaffold proteins into different strains of L. plantarum. The spatial differentiation of the components of the enzymatic systems alleviated the load on the cell machinery of the different ...Continue Reading

Citations

Dec 3, 2014·Current Opinion in Biotechnology·Riaan den HaanWillem H van Zyl
May 14, 2016·Applied Microbiology and Biotechnology·Larisa Cortes-TolalpaJan Dirk van Elsas
Sep 14, 2016·Proceedings of the National Academy of Sciences of the United States of America·Lital DavidiEdward A Bayer
Aug 13, 2017·Biotechnology and Bioengineering·Chiara GandiniRoberto Mazzoli
Sep 1, 2018·FEMS Microbiology Letters·Rajni Hatti-KaulHesham El Enshasy
Jul 11, 2019·International Journal of Molecular Sciences·Ying WangShao-Yuan Leu
Jul 10, 2019·Microbial Ecology·Pilar Eliana Puentes-Téllez, Joana Falcao Salles
Dec 13, 2016·Nature Reviews. Microbiology·Lior ArtziSarah Moraïs
Aug 14, 2019·Biotechnology and Applied Biochemistry·Bin-Bin Hu, Ming-Jun Zhu
Jan 19, 2020·Proceedings of the National Academy of Sciences of the United States of America·Marimuthu AnandharajWen-Hsiung Li
Jun 4, 2019·Chemosphere·Rajesh Kumar PrasadDharmendra Kumar Gupta
Sep 3, 2020·ACS Synthetic Biology·Pavel DvořákVíctor de Lorenzo

❮ Previous
Next ❯

Methods Mentioned

BETA
dot-blot
ELISA
scanning electron microscopy
fluorescence microscopy

Software Mentioned

Protparam

Related Concepts

Related Feeds

Biofuels (ASM)

Biofuels are produced through contemporary processes from biomass rather than geological processes involved in fossil fuel formation. Examples include biodiesel, green diesel, biogas, etc. Discover the latest research on biofuels in this feed.