A combined HIV-1 protein bead array for serology assay and T-cell subset immunophenotyping with a hybrid flow cytometer: a step in the direction of a comprehensive multitasking instrument platform for infectious disease diagnosis and monitoring

Cytometry. Part B, Clinical Cytometry
Sylvie FaucherF F Mandy

Abstract

A new generation of bench-top flow cytometers with digital signal processing to perform suspension array technology (SAT) based bead array assays as well as leukocyte immunophenotyping is now available. These hybrid instruments provide an opportunity for the development of a more cost effective multitasking platform to support infectious disease treatment in resource limited countries. We report the development and testing of two modules compatible with the hybrid flow cytometers. The first module is an eleven HIV-1 protein bead array (PBA) for the detection of circulating antibodies and the second is a cell based T-cell enumeration assay. The HIV-1 PBA was tested in parallel with two enzyme immunoassays (EIAs) for the detection of plasma antibodies from 4 HIV-1 seroconversion panels and a low antibody titer panel. The PBA as well as the two EIAs performed equally for the detection of antibody positive samples from all seroconversion panels. One antibody positive sample from the low antibody titer panel was missed by the PBA together with one of the two EIAs tested. A parallel analysis of the HIV-1 PBA with Western blot (a confirmatory test for HIV infection) using plasma from nine HIV-1(+) individuals showed that the HIV-1 PBA...Continue Reading

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Citations

Nov 8, 2008·Journal of Occupational Medicine and Toxicology·Nasr Ya Hemdan
Feb 5, 2008·Journal of Chromatography. a·Jishan Li, Wenwan Zhong
Mar 1, 2008·Cytometry. Part B, Clinical Cytometry·F MandyS Faucher
Sep 5, 2008·Current Protocols in Cytometry·John P NolanHenri C van der Heyde

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