A Combined Metabolomic and Proteomic Study Revealed the Difference in Metabolite and Protein Expression Profiles in Ruminal Tissue From Goats Fed Hay or High-Grain Diets

Frontiers in Physiology
Changzheng GuoSheng-Yong Mao

Abstract

Currently, knowledge about the impact of high-grain (HG) feeding on metabolite and protein expression profiles in ruminal tissue is limited. In this study, a combination of proteomic and metabolomic approaches was applied to evaluate metabolic and proteomic changes of the rumen epithelium in goats fed a hay diet (Hay) or HG diet. At the metabolome level, results from principal component analysis (PCA) and PLS-DA revealed clear differences in the biochemical composition of ruminal tissue of the control (Hay) and the grain-fed groups, demonstrating the evident impact of HG feeding on metabolite profile of ruminal epithelial tissues. As compared with the Hay group, HG feeding increased the levels of eight metabolites and decreased the concentrations of seven metabolites in ruminal epithelial tissues. HG feeding mainly altered starch and sucrose metabolism, purine metabolism, glyoxylate and dicarboxylate metabolism, glycerolipid metabolism, pyruvate metabolism, glycolysis or gluconeogenesis, galactose metabolism, glycine, serine and threonine metabolism, and arginine and proline metabolism in ruminal epithelium. At the proteome level, 35 differentially expressed proteins were found in the rumen epithelium between the Hay and HG gro...Continue Reading

References

Jun 1, 1985·European Journal of Immunology·J Barankiewicz, A Cohen
Oct 1, 1987·Quarterly Journal of Experimental Physiology : an International Journal of the Physiological Society·G GaebelP Galfi
Oct 1, 1981·Quarterly Journal of Experimental Physiology : an International Journal of the Physiological Society·R A Goodlad
Oct 7, 1994·Cell·A Ciechanover
Nov 14, 1997·The Journal of Clinical Endocrinology and Metabolism·G EisenhoferE Mezey
Oct 29, 2002·Animal Health Research Reviews·G GäbelF Müller
Mar 15, 2003·Biochimica Et Biophysica Acta·Dayin LinJohn R Yates
May 20, 2003·European Journal of Biochemistry·Bernhard GessArmin Kurtz
May 29, 2003·Proceedings of the National Academy of Sciences of the United States of America·Scott A GerberSteven P Gygi
Jul 1, 1956·The Biochemical Journal·R J PENNINGTON, T M SUTHERLAND
Nov 25, 2003·Journal of Animal Physiology and Animal Nutrition·S K UppalH Martens
Jun 29, 2004·Muscle & Nerve·Stefan Probst-CousinDieter Heuss
Apr 19, 2005·Archives of Pharmacal Research·Changjiang XuAh-Ng Tony Kong
Sep 1, 1982·Applied and Environmental Microbiology·A ChessonR J Wallace
Mar 29, 2007·Animal Health Research Reviews·Khalid AbdounHolger Martens
May 31, 2008·Nature Reviews. Drug Discovery·Masato Furuhashi, Gökhan S Hotamisligil
Sep 21, 2010·Applied and Environmental Microbiology·S C FernandoU Desilva
May 14, 2011·American Journal of Physiology. Gastrointestinal and Liver Physiology·Angelika BondzioRalf Einspanier
Jun 7, 2013·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Jun-hua LiuSheng-yong Mao
Dec 18, 2013·Biochimica Et Biophysica Acta·Carlo W T van RoermundHans R Waterham
Aug 22, 2014·Journal of Inherited Metabolic Disease·C Bieneck HaglindM Halldin Stenlid
Dec 10, 2016·Frontiers in Microbiology·André BanninkJan Dijkstra

❮ Previous
Next ❯

Citations

Nov 21, 2020·The Journal of Dairy Research·Sylvie Giger-ReverdinValérie Berthelot
Jan 5, 2021·Frontiers in Genetics·Zhou-Lin WuSong-Jia Lai

❮ Previous
Next ❯

Methods Mentioned

BETA
electrophoresis
dissection
PCA
protein folding

Software Mentioned

SIMCA
MetaboAnalyst
SPSS
OmicsBean
Proteome Discoverer
DA
- P +
ChromAlign
SIEVE

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.