A combined patch-clamp and electrorotation study of the voltage- and frequency-dependent membrane capacitance caused by structurally dissimilar lipophilic anions

The Journal of Membrane Biology
D ZimmermannV L Sukhorukov

Abstract

Interactions of structurally dissimilar anionic compounds with the plasma membrane of HEK293 cells were analyzed by patch clamp and electrorotation. The combined approach provides complementary information on the lipophilicity, preferential affinity of the anions to the inner/outer membrane leaflet, adsorption depth and transmembrane mobility. The anionic species studied here included the well-known lipophilic anions dipicrylamine (DPA(-)), tetraphenylborate (TPB(-)) and [W(2)(CO)(10)(S(2)CH)](-), the putative lipophilic anion B(CF(3))(4)(-) and three new heterocyclic W(CO)(5) derivatives. All tested anions partitioned strongly into the cell membrane, as indicated by the capacitance increase in patch-clamped cells. The capacitance increment exhibited a bell-shaped dependence on membrane voltage. The midpoint potentials of the maximum capacitance increment were negative, indicating the exclusion of lipophilic anions from the outer membrane leaflet. The adsorption depth of the large organic anions DPA(-), TPB(-) and B(CF(3))(4)(-) increased and that of W(CO)(5) derivatives decreased with increasing concentration of mobile charges. In agreement with the patch-clamp data, electrorotation of cells treated with DPA(-) and W(CO)(5) de...Continue Reading

References

Jan 1, 1978·Biophysical Journal·O S AndersenS McLaughlin
Dec 1, 1978·Biophysical Journal·C C Wang, L J Bruner
Jul 7, 1988·Biochimica Et Biophysica Acta·W M ArnoldJ Ahlers
Jan 1, 1985·The Journal of Membrane Biology·J P Dilger, R Benz
Nov 1, 1981·Quarterly Reviews of Biophysics·P LäugerW Brock
Sep 1, 1983·The Journal of General Physiology·J M FernándezF Bezanilla
Aug 24, 1983·Biochimica Et Biophysica Acta·A D Pickar, W C Brown
Nov 21, 1995·Proceedings of the National Academy of Sciences of the United States of America·C C LuD W Hilgemann
Apr 1, 1994·Biophysical Journal·E StefaniF Bezanilla
Aug 1, 1995·Biophysical Journal·A F Oberhauser, J M Fernandez
Jan 24, 1998·The Journal of Biological Chemistry·G NagelH Koepsell
Sep 3, 1998·Applied and Environmental Microbiology·H De WeverH Verachtert
Dec 5, 1998·The Journal of Membrane Biology·M Wu, J Santos-Sacchi
May 23, 2002·Biophysical Journal·Jens Schamberger, Ronald J Clarke
May 24, 2003·Annals of the New York Academy of Sciences·Irena Klodos
Nov 22, 2005·Biophysical Journal·Enrique G Navarrete, Joseph Santos-Sacchi
Sep 1, 1971·The Journal of Membrane Biology·B KettererP Läuger

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Citations

May 22, 2008·The Journal of Membrane Biology·Y N AntonenkoV P Skulachev
Sep 23, 2008·The Journal of Membrane Biology·Tatyana I RokitskayaYuri N Antonenko
Aug 6, 2008·Brain Cell Biology·B J BakerT Knöpfel
Jun 18, 2014·The Journal of Membrane Biology·Yuta Hidaka, Koji Asami
May 20, 2009·Biophysical Journal·Walther AkemannThomas Knöpfel
Jun 1, 2010·Biochimica Et Biophysica Acta·Tatyana I RokitskayaVladimir P Skulachev
Sep 13, 2014·Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry·Toshio TakayanagiTomoki Yabutani
Apr 23, 2013·Biophysical Journal·Amal El-GaddarL Krähenbühl
Mar 5, 2019·Electrophoresis·Marion CostellaMichael Canva
Apr 17, 2020·Analytical and Bioanalytical Chemistry·Mohamed Z Rashed, Stuart J Williams
Apr 25, 2014·Langmuir : the ACS Journal of Surfaces and Colloids·Samia MenadMarie Frenea-Robin

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Methods Mentioned

BETA
X-ray
glycosylation

Software Mentioned

Origin
ROT
Clampex
Clampfit

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