A combined vaccine approach against Vibrio cholerae and ETEC based on outer membrane vesicles

Frontiers in Microbiology
Deborah R LeitnerStefan Schild

Abstract

Enteric infections induced by pathogens like Vibrio cholerae and enterotoxigenic Escherichia coli (ETEC) remain a massive burden in developing countries with increasing morbidity and mortality rates. Previously, we showed that the immunization with genetically detoxified outer membrane vesicles (OMVs) derived from V. cholerae elicits a protective immune response based on the generation of O antigen antibodies, which effectively block the motility by binding to the sheathed flagellum. In this study, we investigated the potential of lipopolysaccharide (LPS)-modified and toxin negative OMVs isolated from V. cholerae and ETEC as a combined OMV vaccine candidate. Our results indicate that the immunization with V. cholerae or ETEC OMVs induced a species-specific immune response, whereas the combination of both OMV species resulted in a high-titer, protective immune response against both pathogens. Interestingly, the immunization with V. cholerae OMVs alone resulted in a so far uncharacterized and cholera toxin B-subunit (CTB) independent protection mechanism against an ETEC colonization. Furthermore, we investigated the potential use of V. cholerae OMVs as delivery vehicles for the heterologously expression of the ETEC surface antige...Continue Reading

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Citations

Jan 1, 2016·Molecular Membrane Biology·Deepak Anand, Arunima Chaudhuri
Mar 22, 2018·Emerging Microbes & Infections·Noha M Elhosseiny, Ahmed S Attia
Jan 26, 2016·Nature Communications·Sandro RoierStefan Schild
May 10, 2019·F1000Research·Brian Y Hsueh, Christopher M Waters
Mar 27, 2020·Frontiers in Cellular and Infection Microbiology·Christian Rueter, Martina Bielaszewska
Apr 5, 2018·Frontiers in Microbiology·Maricarmen Rojas-LopezRoberto Rosini
Sep 6, 2018·The Journal of Infectious Diseases·Jason B Harris
May 6, 2019·International Journal of Molecular Sciences·Daniel HatlemJack C Leo
Apr 27, 2021·Nanomedicine : Nanotechnology, Biology, and Medicine·Yingying GanXin Ding
Jan 27, 2019·Microbiology Spectrum·Jessica D CecilAnne Marie Krachler
Nov 23, 2021·Frontiers in Microbiology·Himadri B ThapaStefan Schild

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Methods Mentioned

BETA
PCR
dissection
ELISA
electrophoresis
motility assay
motility
fluorescence microscopy

Software Mentioned

GraphPad Prism

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