A common aberration with water-immersion objective lenses

Journal of Microscopy
R Arimoto, J M Murray

Abstract

We describe here an aberration that is frequently encountered with water-immersion but not oil-immersion objectives. The aberration is shown to be induced by tilt of the coverslip out of the plane normal to the optical axis. Model calculations taking into account the path-length distortions introduced by a tilted coverslip satisfactorily reproduce the observed effect on the images of small subresolution fluorescent beads.

Citations

Feb 6, 2014·Histochemistry and Cell Biology·Teresa KleinMarkus Sauer
Feb 3, 2016·Nature Communications·Ulrike BöhmRoman Schmidt
Sep 13, 2013·Journal of Neuroscience Methods·Sadahiro IwabuchiN Charles Harata
May 18, 2016·Micron : the International Research and Review Journal for Microscopy·Yilun Fan, Andrew P Bradley
Jan 11, 2017·Chemical Reviews·Toru KondoGabriela S Schlau-Cohen
Apr 4, 2020·Annual Review of Biomedical Engineering·Sheng LiuFang Huang
Aug 13, 2011·Biomedical Optics Express·Yann CotteChristian Depeursinge
Sep 1, 2017·Biomedical Optics Express·Raphaël TurcotteNa Ji
Oct 14, 2017·Biomedical Optics Express·Per Niklas HeddeEnrico Gratton
Mar 7, 2019·Light, Science & Applications·Craig R CopelandSamuel M Stavis
Aug 27, 2021·Current Protocols·Jessica Valli, Jeremy Sanderson

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